TRPV4-mediated calcium signaling in mesenchymal stem cells regulates aligned collagen matrix formation and vinculin tension
| Autor: | Wolfgang Liedtke, Brenton D. Hoffman, Natasha Case, Holly A. Leddy, Dianne Little, Farshid Guilak, Christopher L. Gilchrist, Katheryn E. Rothenberg, Laurel Kaye |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
TRPV Cation Channels Bone Marrow Cells Matrix (biology) Cell-Matrix Junctions Focal adhesion 03 medical and health sciences Mechanobiology 0302 clinical medicine Humans Calcium Signaling Mechanotransduction Calcium signaling Focal Adhesions Multidisciplinary biology Chemistry Mesenchymal stem cell Mesenchymal Stem Cells Vinculin Extracellular Matrix Cell biology 030104 developmental biology Cellular Microenvironment Physical Sciences biology.protein Calcium Mechanosensitive channels Collagen 030217 neurology & neurosurgery |
| Zdroj: | Proceedings of the National Academy of Sciences. 116:1992-1997 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1811095116 |
| Popis: | Microarchitectural cues drive aligned fibrillar collagen deposition in vivo and in biomaterial scaffolds, but the cell-signaling events that underlie this process are not well understood. Utilizing a multicellular patterning model system that allows for observation of intracellular signaling events during collagen matrix assembly, we investigated the role of calcium (Ca(2+)) signaling in human mesenchymal stem cells (MSCs) during this process. We observed spontaneous Ca(2+) oscillations in MSCs during fibrillar collagen assembly, and hypothesized that the transient receptor potential vanilloid 4 (TRPV4) ion channel, a mechanosensitive Ca(2+)-permeable channel, may regulate this signaling. Inhibition of TRPV4 nearly abolished Ca(2+) signaling at initial stages of collagen matrix assembly, while at later times had reduced but significant effects. Importantly, blocking TRPV4 activity dramatically reduced aligned collagen fibril assembly; conversely, activating TRPV4 accelerated aligned collagen formation. TRPV4-dependent Ca(2+) oscillations were found to be independent of pattern shape or subpattern cell location, suggesting this signaling mechanism is necessary for aligned collagen formation but not sufficient in the absence of physical (microarchitectural) cues that force multicellular alignment. As cell-generated mechanical forces are known to be critical to the matrix assembly process, we examined the role of TRPV4-mediated Ca(2+) signaling in force generated across the load-bearing focal adhesion protein vinculin within MSCs using an FRET-based tension sensor. Inhibiting TRPV4 decreased tensile force across vinculin, whereas TRPV4 activation caused a dynamic unloading and reloading of vinculin. Together, these findings suggest TRPV4 activity regulates forces at cell-matrix adhesions and is critical to aligned collagen matrix assembly by MSCs. |
| Databáze: | OpenAIRE |
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