Cholesterol esterification by host and parasite is essential for optimal proliferation of Toxoplasma gondii
| Autor: | Kami Kim, Sabrina Sonda, Jacquelyn J. Maher, Joel D. Ernst, Sabine Novak, Robert V. Farese, Li Min Ting |
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| Rok vydání: | 2001 |
| Předmět: |
Sterol O-acyltransferase
Endocytic cycle Biochemistry chemistry.chemical_compound Mice parasitic diseases Parasite hosting Animals Humans Anilides Enzyme Inhibitors Molecular Biology chemistry.chemical_classification Host cell membrane biology Cholesterol Toxoplasma gondii Cell Biology biology.organism_classification Cell biology Enzyme chemistry Receptors LDL lipids (amino acids peptides and proteins) Cholesterol Esters Toxoplasma Intracellular Sterol O-Acyltransferase |
| Zdroj: | The Journal of biological chemistry. 276(37) |
| ISSN: | 0021-9258 |
| Popis: | Upon host cell invasion the apicomplexan parasite Toxoplasma gondii resides in a specialized compartment termed the parasitophorous vacuole that is derived from the host cell membrane but modified by the parasite. Despite the segregation of the parasitophorous vacuole from the host endocytic network, the intravacuolar parasite has been shown to acquire cholesterol from the host cell. In order to characterize further the role of sterol metabolism in T. gondii biology, we focused our studies on the activity of acyl-CoA:cholesterol acyltransferase (ACAT), a key enzyme for maintaining the intracellular homeostasis of cholesterol through the formation of cholesterol esters. In this study, we demonstrate that ACAT and cholesterol esters play a crucial role in the optimal replication of T. gondii. Moreover, we identified ACAT activity in T. gondii that can be modulated by pharmacological ACAT inhibitors with a consequent detrimental effect on parasite replication. |
| Databáze: | OpenAIRE |
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