An Ex Vivo Evaluation of Moxifloxacin Nanostructured Lipid Carrier Enriched In Situ Gel for Transcorneal Permeation on Goat Cornea
| Autor: | Krishna Kumar Patel, Sanjay Singh, Ashish Kumar Agrawal, Md. Meraj Anjum, Deepika Deepika, Shilpkala Gade, Chandan Kumar Gupta |
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| Rok vydání: | 2018 |
| Předmět: |
Staphylococcus aureus
Alginates Kinetics Dispersity Moxifloxacin Pharmaceutical Science Administration Ophthalmic 02 engineering and technology Microbial Sensitivity Tests 030226 pharmacology & pharmacy Permeability Cornea 03 medical and health sciences 0302 clinical medicine Differential scanning calorimetry Hypromellose Derivatives Drug Stability X-Ray Diffraction medicine Animals Humans Drug Carriers Endophthalmitis Chromatography Calorimetry Differential Scanning Chemistry Goats Permeation 021001 nanoscience & nanotechnology Lipids Anti-Bacterial Agents Drug Liberation medicine.anatomical_structure Nanoparticles Particle size 0210 nano-technology Gels Ex vivo medicine.drug |
| Zdroj: | Journal of pharmaceutical sciences. 108(9) |
| ISSN: | 1520-6017 |
| Popis: | The study was designed to fabricate the moxifloxacin nanostructured lipid carriers (MOX-NLCs) loaded in situ gel for opthalmic application to improve the corneal permeation and retention and also subside the toxic effect associated with intracameral injection of moxifloxacin in endophthalmitis treatment. Initially, Box-Behnken design was used to optimize the various factors significantly affecting the final formulation attributes. MOX-NLCs with particle size 232.1 ± 9.2 nm, polydispersity index 0.247 ± 0.031, zeta potential −16.3 ± 1.6 mV, entrapment efficiency 63.1 ± 2.4%, and spherical shape was achieved. The optimized MOX-NLCs demonstrated the Higuchi release kinetics with highest regression coefficient. Besides this, FTIR, differential scanning calorimetry, and X-ray diffraction results suggested that MOX had excellent compatibility with excipients. Furthermore, the results of ex-vivo permeation study demonstrated 2-fold higher permeation (208.7 ± 17.6 μg), retention (37.26 ± 2.83 μg), and flux (9.57 ± 0.73 μg/cm2 h) compared with free MOX in situ gel. In addition, MOX-NLCs exhibited normal corneal hydration and did not show any sign of structural damage to the corneal tissue as confirmed by histology. Therefore, the findings strongly suggest that MOX-NLCs in situ gel with higher permeation and retention can be a better alternative strategy to prevent and treat the endophthalmitis infection. |
| Databáze: | OpenAIRE |
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