Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources
| Autor: | Sherif M. El-Kadi, Hossam F. A. EL-Shaer, Mohssen Elbagory, Dina Fathi Ismail Ali, Sahar El-Nahrawy, Hassan A. H. EL-Zawawy, Alaa El-Dein Omara, Adel A. Shoukry |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Polymers and Plastics
Microorganism PHB chemistry.chemical_element Organic chemistry macromolecular substances medicine.disease_cause Article chemistry.chemical_compound QD241-441 Biosynthesis Dry weight Azotobacter salinestris medicine Food science IR spectrometry Chemical composition GC-MS/MS spectrometry biology agro-industrial waste technology industry and agriculture General Chemistry biology.organism_classification chemistry Sugar beet lipids (amino acids peptides and proteins) Carbon Isopropyl |
| Zdroj: | Polymers, Vol 13, Iss 3801, p 3801 (2021) Polymers Volume 13 Issue 21 |
| ISSN: | 2073-4360 |
| Popis: | Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were Bacillus paramycoides MCCC 1A04098, Azotobacter salinestris NBRC 102611 and Brevundimonas naejangsanensis BIO-TAS2-2. The addition of sugar beet molasses to the medium of A. salinestris increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the produced PHB from B. paramycoides and A. salinestris showed similar bands which confirmed the presence of PHB however, B. naejangsanensis showed weak bands, indicating lower PHB concentration. The chemical composition obtained showed that the GC-MS of the PHB extracted represents 2, 4-ditert-butylphenol for B. paramycoides and isopropyl ester of 2-butenoic acid for both of A. salinestris and Brevundimonas naejangsanensis. Therefore, PHB produced by microorganisms can be considered a biodegradable polyester, and represents a promising technique for the development of eco-friendly and fully biodegradable plastics. |
| Databáze: | OpenAIRE |
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