Autopsy Study of Calretinin Immunohistochemistry in the Anorectal Canal in Young Infants and Potential Implications for Rectal Biopsy Approach in the Neonatal Period
| Autor: | Zachary J Dureau, Heather Rytting, Jose Velazquez Vega, Beverly Barton Rogers, Hong Yin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Period (gene) Biopsy Rectal biopsy Autopsy Pathology and Forensic Medicine Young infants 03 medical and health sciences 0302 clinical medicine Medicine Humans Hirschsprung Disease business.industry Infant Newborn Rectum Infant General Medicine Pectinate line Immunohistochemistry Ganglion medicine.anatomical_structure nervous system 030220 oncology & carcinogenesis Calbindin 2 Pediatrics Perinatology and Child Health 030211 gastroenterology & hepatology Calretinin business |
| Zdroj: | Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society. 24(6) |
| ISSN: | 1615-5742 |
| Popis: | Background Absent submucosal ganglion cells in biopsies 1-3 cm above the pectinate line establishes the pathologic diagnosis of Hirschsprung Disease (HD). Calretinin stains both ganglion cells and their mucosal neurites and has gained importance in HD diagnosis. Absent calretinin positive mucosal neurites in biopsies at the appropriate level above the pectinate line is highly specific for HD. Whether this applies to lower biopsies is uncertain. To address this, we studied anorectal canal autopsy specimens from infants. Methods We performed an autopsy study of infant anorectal canal specimens to describe calretinin staining in this region. Calretinin staining was correlated with histologic and gross landmarks. Results In all 15 non-HD specimens, calretinin positive mucosal neurites were present in glandular mucosa up to the anorectal line where neurites rapidly diminished. Age range was preterm 26 weeks to 3 months. Conclusions Calretinin positive mucosal neurites are present in glandular mucosa up to the anorectal line in young infants. This is potentially important regarding neonatal HD biopsy level and diagnosis. Positive calretinin staining at the anorectal line favors normal innervation making HD unlikely. Absent calretinin positive neurites in glandular mucosa is worrisome for HD in young infants, regardless of location. |
| Databáze: | OpenAIRE |
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