Structure-function relationship of a citrus salicylate methylesterase and role of salicylic acid in citrus canker resistance
| Autor: | Gustavo Fernando Mercaldi, Celso Eduardo Benedetti, Hugo Shimo, Caio Cesar de Lima Silva, Rafael de Felício, Silvana A. Rocco |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Article Xanthomonas citri 03 medical and health sciences chemistry.chemical_compound Structure-Activity Relationship 0302 clinical medicine Xanthomonas medicine lcsh:Science Disease Resistance Plant Diseases Plant Proteins Canker Multidisciplinary biology lcsh:R food and beverages biology.organism_classification medicine.disease Salicylates 030104 developmental biology chemistry Biochemistry Citrus canker lcsh:Q 030217 neurology & neurosurgery Methyl salicylate Systemic acquired resistance Citrus × sinensis Salicylic acid Citrus sinensis |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that the Citrus sinensis SABP2 homologue protein CsMES1 catalyzes the hydrolysis of MeSA into SA. Molecular modeling studies suggest that CsMES1 shares the same structure and SA-binding mode with tobacco SABP2. However, an amino acid polymorphism in the active site of CsMES1-related proteins suggested an important role in enzyme regulation. We present evidence that the side chain of this polymorphic residue directly influences enzyme activity and SA binding affinity in CsMES proteins. We also show that SA and CsMES1 transcripts preferentially accumulate during the incompatible interaction between Xanthomonas aurantifolii pathotype C and sweet orange plants. Moreover, we demonstrate that SA and MeSA inhibited citrus canker caused by Xanthomonas citri, whereas an inhibitor of CsMES1 enhanced canker formation, suggesting that CsMES1 and SA play a role in the local defense against citrus canker bacteria. |
| Databáze: | OpenAIRE |
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