An Engineered Constitutive Promoter Set with Broad Activity Range for Cupriavidus necator H16
| Autor: | Miriam Gonzalez-Villanueva, Abayomi Oluwanbe Johnson, Tuck Seng Wong, Kang Lan Tee |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cupriavidus necator Point mutation Saccharomyces cerevisiae Biomedical Engineering Promoter General Medicine Computational biology Biology biology.organism_classification medicine.disease_cause Biochemistry Genetics and Molecular Biology (miscellaneous) Metabolic engineering 03 medical and health sciences Synthetic biology 030104 developmental biology Bacterial Proteins Escherichia coli medicine Synthetic Biology Biomanufacturing Promoter Regions Genetic Plasmids |
| Zdroj: | ACS Synthetic Biology. 7:1918-1928 |
| ISSN: | 2161-5063 |
| Popis: | Well-characterized promoters with variable strength form the foundation of heterologous pathway optimization. It is also a key element that bolsters the success of microbial engineering and facilitates the development of biological tools like biosensors. In comparison to microbial hosts such as Escherichia coli and Saccharomyces cerevisiae, the promoter repertoire of Cupriavidus necator H16 is highly limited. This limited number of characterized promoters poses a significant challenge during the engineering of C. necator H16 for biomanufacturing and biotechnological applications. In this article, we first examined the architecture and genetic elements of the four most widely used constitutive promoters of C. necator H16 (i.e., PphaC1, PrrsC, Pj5, and Pg25) and established a narrow 6-fold difference in their promoter activities. Next, using these four promoters as starting points and applying a range of genetic modifications (including point mutation, length alteration, incorporation of regulatory genetic ... |
| Databáze: | OpenAIRE |
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