Embryonic Stem Cell Proliferation Stimulated By Altered Anabolic Metabolism From Glucose Transporter 2-Transported Glucosamine
| Autor: | Jin Hyuk Jung, Kumiko Iwabuchi, Mary R. Loeken, Zhihong Yang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male endocrine system Glycosylation Biology digestive system Article Cell Line 03 medical and health sciences chemistry.chemical_compound Mice Glucosamine Animals Cell Proliferation Glucose Transporter Type 2 Multidisciplinary 030102 biochemistry & molecular biology Cell growth Transporter Biological Transport Mouse Embryonic Stem Cells Metabolism Embryonic stem cell Glutamine carbohydrates (lipids) 030104 developmental biology chemistry Biochemistry Cell culture embryonic structures biology.protein GLUT2 lipids (amino acids peptides and proteins) Female Protein Processing Post-Translational Metabolic Networks and Pathways Transcription Factors |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The hexose transporter, GLUT2 (SLC2A2), which is expressed by mouse embryos, is important for survival before embryonic day 10.5, but its function in embryos is unknown. GLUT2 can transport the amino sugar glucosamine (GlcN), which could increase substrate for the hexosamine biosynthetic pathway (HBSP) that produces UDP-N-acetylglucosamine for O-linked N-acetylglucosamine modification (O-GlcNAcylation) of proteins. To understand this, we employed a novel murine embryonic stem cell (ESC) line that, like mouse embryos, expresses functional GLUT2 transporters. GlcN stimulated ESC proliferation in a GLUT2-dependent fashion but did not regulate pluripotency. Stimulation of proliferation was not due to increased O-GlcNAcylation. Instead, GlcN decreased dependence of the HBSP on fructose-6-PO4 and glutamine. Consequently, glycolytic- and glutamine-derived intermediates that are needed for anabolic metabolism were increased. Thus, maternally obtained GlcN may increase substrates for biomass accumulation by embryos, as exogenous GlcN does for GLUT2-expressing ESC and may explain the need for GLUT2 expression by embryos. |
| Databáze: | OpenAIRE |
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