Detection of Polymorphism in Puccinia hordei using RFLP and RAPD Markers, Differential Cultivars, and Analysis of the Intergenic Spacer Region of rDNA

Autor:	K. W. Buck, J. M. Jennings, Adrian C. Newton
Rok vydání:	1997
Předmět:	Genetics education.field_of_study biology Physiology Population food and beverages Plant Science Spacer DNA biology.organism_classification RAPD parasitic diseases Genotype Hordeum vulgare Restriction fragment length polymorphism Puccinia hordei education Agronomy and Crop Science Ribosomal DNA
Zdroj:	Scopus-Elsevier
ISSN:	1439-0434 0931-1785
Popis:	A world-wide range of isolates of Puccinia hordei and P. striiformis f. sp. hordei and UK isolates of P. striiformis f. sp. tritici and P. recondita were characterized using DNA restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), ribosomal DNA (rDNA) intergenic sequence (IGS) or virulence on differential cultivars. The different species were readily distinguished by both RFLPs and RAPDs. Within a species, variability was detected using differential cultivars, RAPDs, and RFLPs with probes from the rDNA region, indicating their potential for population analysis. However, RFLPs within a species were not detected using a wide range of random (anonymous) probes from a P. hordei genomic library. Previous assumptions about the general uniformity of rust genotypes were reassessed from these data.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3953a09d1f251bfe5f4329aa5e55398 https://doi.org/10.1111/j.1439-0434.1997.tb00359.x Zobrazit plný text záznamu Plný text ve formátu PDF