A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates of Corynebacterium group D2
| Autor: | D. G. Pitcher, A. Soto, Francisco Soriano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
DNA
Bacterial Bacteriuria Epidemiology Restriction Mapping Corynebacterium DNA Ribosomal Restriction fragment Ribotyping Restriction map Cluster Analysis Humans Typing Gene Genetics biology Computers RNA Probes Ribosomal RNA biology.organism_classification Molecular biology Bacterial Typing Techniques RNA Bacterial Infectious Diseases RNA Ribosomal biology.protein Restriction digest Research Article |
| Popis: | SUMMARYRestriction digest fragments of DNA from 46 clinical isolates identified asCorynebacteriumgroup D2, were separated by electrophoresis, Southern blotted onto nylon membranes and hybridized to a ribosomal RNA gene probe. The resulting band patterns were subjected to unweighed pair-group cluster analysis. Representative strains from the main clusters were compared with similarly prepared band patterns from type strains of humanCorynebacteriumspecies.The results indicate that strains identified asCorynebacteriumgroup D2 represent a unique taxon and that computer-assisted analysis of rRNA gene restriction fragment polymorphism (ribotyping) could be a useful technique in epidemiological studies of these bacteria. |
| Databáze: | OpenAIRE |
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