Probing the Antitumor Mechanism of Solanum nigrum L. Aqueous Extract against Human Breast Cancer MCF7 Cells
| Autor: | Jinha Yang, Shujun Xiao, Jian Yang, Wei Ying, Binbing Ling, Meena Kishore Sakharkar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
cell migration
Solanum nigrum L Bioengineering Pharmacology Biology Solanum nigrum gene-disease association network 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine breast cancer gene-drug interaction network cytotoxicity glycolysis medicine Glycolysis Cytotoxicity Gene 030304 developmental biology 0303 health sciences Hexokinase Cancer Cell migration medicine.disease biology.organism_classification chemistry 030220 oncology & carcinogenesis Pyruvate kinase |
| Zdroj: | Bioengineering Volume 6 Issue 4 Bioengineering, 6(4):112 |
| ISSN: | 2306-5354 |
| DOI: | 10.3390/bioengineering6040112 |
| Popis: | Solanum nigrum L. is one of the major medicinal plants used to treat cancer. However, the functional mechanism of S. nigrum L. extract is still unknown in spite of numerous studies on its active components. In this study, we probed the potential anticancer mechanism of the aqueous extract of S. nigrum L. (AESN) towards human breast cancer cell line MCF7. At a concentration of 10 g/L, AESN caused 43% cytotoxicity, inhibited the migration, and suppressed the activities of hexokinase and pyruvate kinase by about 30% and 40%, respectively, towards the MCF7 cells. RT2-PCR analysis of a panel of 89 caner-related genes identified 13 upregulated and eight downregulated genes (> 2-folds) in MCF7 cells upon AESN treatment. Gene ontology (GO) and functional disease ontology (FunDO) analyses show that the antitumor function of S. nigrum L. involves multiple genes and these genes are shared across other diseases or disorders. |
| Databáze: | OpenAIRE |
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