Maturational Changes in Na+-K+Pump Activity in Guinea Pig Airway Smooth Muscle
| Autor: | Kerry J. Rhoden, Magdalena Souhrada, James S. Douglas |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pulmonary and Respiratory Medicine
Aging Sodium-Potassium-Exchanging ATPase Sodium Guinea Pigs Clinical Biochemistry chemistry.chemical_element Diaphragm pump Biology Muscle Development Ouabain Guinea pig medicine Animals Enzyme Inhibitors Na+/K+-ATPase Molecular Biology Membrane potential Cell Membrane Biological Transport Muscle Smooth respiratory system Trachea Animals Newborn Biochemistry chemistry Potassium Biophysics Female Rubidium Radioisotopes Intracellular medicine.drug |
| Zdroj: | Experimental Lung Research. 23:333-345 |
| ISSN: | 1521-0499 0190-2148 |
| DOI: | 10.3109/01902149709039230 |
| Popis: | The effect of maturation on the Na(+)-K+ pump of airway smooth muscle (ASM) was studied. Na(+)-K+ pump activity of tracheal smooth muscle from immature (1- to 2-week-old) and mature (10- to 12-week-old) guinea pigs was measured as the ouabain-sensitive component of the resting membrane potential and as ouabain-sensitive 86Rb+ uptake (using 86Rb+ as a marker for K+). Maturation resulted in decreases in both the contribution of the Na(+)-K+ pump of the resting membrane potential and in ouabain-sensitive 86Rb+ uptake. 86Rb+ efflux from tracheal smooth muscle was similar in tissues from immature and mature guinea pigs, suggesting that maturation has no effect on K+ (or at least 86Rb+) permeability. Na+ and K+ contents of tracheal smooth muscle were estimated from the uptakes of 24Na+ and 86Rb+ at equilibrium. Maturation resulted in a decrease in Na+ content but had no effect on K+ content. Since intracellular Na+ is one of the principal determinants of Na(+)-K+ pump activity, these results suggest that maturation results in a decrease in Na(+)-K+ pump activity in ASM, which may be due to a decrease in Na+ content. |
| Databáze: | OpenAIRE |
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