Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability
| Autor: | Terry W. J. Steele, Joachim Say Chye Loo, Raphael Ortiz, Raghavendra C. Mundargi, Subbu S. Venkatraman, Saranya Kumar, Vishal Mogal, Divya Venkataraman |
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| Přispěvatelé: | School of Materials Science & Engineering |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Endothelium Article Subject Swine H&E stain lcsh:Medicine General Biochemistry Genetics and Molecular Biology Masson's trichrome stain Bioreactors Tissue engineering In vivo Bioreactor medicine Pressure Animals Vascular tissue General Immunology and Microbiology Tissue Engineering Chemistry lcsh:R technology industry and agriculture Temperature General Medicine Equipment Design equipment and supplies medicine.anatomical_structure Carotid Arteries Female Ex vivo Biomedical engineering Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2015 (2015) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | The aim of the present work is to design and construct anex vivobioreactor system to assess the real time viability of vascular tissue. Porcine carotid artery as a model tissue was used in theex vivobioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure, temperature, and flow. The real time tissue viability was evaluated by monitoring tissue metabolism through a fluorescent indicator “resorufin.” Ourex vivobioreactor allows real time monitoring of tissue responses along with physiological conditions. Theseex vivoparameters were vital in determining the tissue viability in sensor-enabled bioreactor and our initial investigations suggest that, porcine tissue viability is considerably affected by high shear forces and low oxygen levels. Histological evaluations with hematoxylin and eosin and Masson’s trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation inex vivobioreactor studies indicate denuded endothelium supporting the viability results from real time measurements. Hence, this novel viability sensor-enabledex vivobioreactor acts as model to mimicin vivosystem and record vascular responses to biopharmaceutical molecules and biomedical devices. |
| Databáze: | OpenAIRE |
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