Endocytotic elimination and domain-selective tethering constitute a potential mechanism of protein segregation at the axonal initial segment
| Autor: | Bénédicte Dargent, Marie-Pierre Fache, Juan José Garrido, Anissa Moussif, Pierre Giraud, Fanny Fernandes |
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| Rok vydání: | 2004 |
| Předmět: |
neuronal polarity
Cytoplasm Time Factors Amino Acid Motifs Detergents Molecular Sequence Data Population axonal initial segment ankyrin G tethering sorting sodium channels Glutamic Acid Biology Transfection Endocytosis Hippocampus Article medicine Animals Point Mutation Amino Acid Sequence Axon education Cytoskeleton Research Articles Neurons education.field_of_study Brefeldin A Microscopy Confocal Sodium channel DNA Cell Biology Molecular biology Axons Protein Structure Tertiary Rats Cell biology Kinetics medicine.anatomical_structure Microscopy Fluorescence nervous system CD4 Antigens COS Cells Soma Neuron |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | The axonal initial segment is a unique subdomain of the neuron that maintains cellular polarization and contributes to electrogenesis. To obtain new insights into the mechanisms that determine protein segregation in this subdomain, we analyzed the trafficking of a reporter protein containing the cytoplasmic II-III linker sequence involved in sodium channel targeting and clustering. Here, we show that this reporter protein is preferentially inserted in the somatodendritic domain and is trapped at the axonal initial segment by tethering to the cytoskeleton, before its insertion in the axonal tips. The nontethered population in dendrites, soma, and the distal part of axons is subsequently eliminated by endocytosis. We provide evidence for the involvement of two independent determinants in the II-III linker of sodium channels. These findings indicate that endocytotic elimination and domain-selective tethering constitute a potential mechanism of protein segregation at the axonal initial segment of hippocampal neurons. |
| Databáze: | OpenAIRE |
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