Validation of a real-time PCR assay for detection of swinepox virus
| Autor: | Anselmo Vasconcelos Rivetti Júnior, Clarissa R. Damaso, Mateus Laguardia-Nascimento, Erlânio Marcelo Dos Santos Júnior, Tânia R.P. Freitas, Antônio Augusto Fonseca Júnior, Felipe Augusto de Souza, Marcelo Fernandes Camargos |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Swine Family Poxviridae Suipoxvirus Poxviridae Infections Biology Real-Time Polymerase Chain Reaction Virus 03 medical and health sciences High morbidity Medical microbiology Limit of Detection Virology medicine Animals 030304 developmental biology Swine Diseases Detection limit 0303 health sciences 030306 microbiology General Medicine Repeatability Real-time polymerase chain reaction DNA Viral Nucleic acid |
| Zdroj: | Archives of Virology. 164:3059-3063 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Swine are the only known hosts of swinepox virus (SWPV), the sole member of the genus Suipoxvirus, family Poxviridae. Rapid diagnosis is recommended for appropriate interventions because of the high morbidity associated with this virus. This study describes a real-time quantitative PCR (qPCR) assay for rapid detection and quantification of SWPV. The detection limit, repeatability, reproducibility, and specificity of this assay were determined. The efficiency was 96%, and the R2 value was 0.996. The detection limit was 1 fg or 10-0.5 TCID50/50 μL. Tests showed that the greatest source of error in the SWPV qPCR assay was variation between analysts rather than different qPCR kits or equipment. All nucleic acids from other viruses or samples collected from swine were negative in the specificity test. qPCR for SWPV is a new method with tested variables that allows main sources of error in laboratory diagnosis and viral quantification to be identified. |
| Databáze: | OpenAIRE |
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