Challenges for the self-assembly of poly(Ethylene Glycol)–poly(Lactic acid) (PEG-PLA) into polymersomes: beyond the theoretical paradigms
| Autor: | Carlota de Oliveira Rangel-Yagui, Alexsandra Conceição Apolinário, Adalberto Pessoa, Monika S. Magon |
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| Přispěvatelé: | University of St Andrews. School of Biology, University of St Andrews. Biomedical Sciences Research Complex |
| Rok vydání: | 2018 |
| Předmět: |
post-film hydration steps
General Chemical Engineering amphiphilic block copolymers Amphiphilic block copolymers NDAS polymeric vesicles self-aggregated nanostructures 02 engineering and technology 010402 general chemistry 01 natural sciences Article lcsh:Chemistry chemistry.chemical_compound Materials Science(all) Amphiphile Copolymer QD General Materials Science Post-film hydration steps Aqueous solution Vesicle QD Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Lactic acid Self-aggregated nanostructures lcsh:QD1-999 chemistry Chemical engineering Polymeric vesicles Polymersome Chemical Engineering(all) Extrusion 0210 nano-technology HIDRATAÇÃO Ethylene glycol |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Nanomaterials; Volume 8; Issue 6; Pages: 373 Nanomaterials, Vol 8, Iss 6, p 373 (2018) Nanomaterials |
| Popis: | We acknowledge support from the State of São Paulo Research Foundation (FAPESP-Brazil, projects 2013/08617-7 (Thematic project) and 2014/10456-4 (Apolinário, A.C. PhD fellowship) and the National Council for Scientific and Technological Development (CNPq- Brazil, project 303334/2014-2). Polymersomes (PL), vesicles formed by self-assembly of amphiphilic block copolymers, have been described as promising nanosystems for drug delivery, especially of biomolecules. The film hydration method (FH) is widely used for PL preparation, however, it often requires long hydration times and commonly results in broad size distribution. In this work, we describe the challenges of the self-assembly of poly (ethylene glycol)-poly(lactic acid) (PEG-PLA) into PL by FH exploring different hydrophilic volume fraction (f) values of this copolymer, stirring times, temperatures and post-FH steps in an attempt to reduce broad size distribution of the nanostructures. We demonstrate that, alongside f value, the methods employed for hydration and post-film steps influence the PEG-PLA self-assembly into PL. With initial FH, we found high PDI values (>0.4). However, post-hydration centrifugation significantly reduced PDI to 0.280. Moreover, extrusion at higher concentrations resulted in further improvement of the monodispersity of the samples and narrow size distribution. For PL prepared at concentration of 0.1% (m/v), extrusion resulted in the narrower size distributions corresponding to PDI values of 0.345, 0.144 and 0.081 for PEG45-PLA69, PEG114-PLA153 and PEG114-PLA180, respectively. Additionally, we demonstrated that copolymers with smaller f resulted in larger PL and, therefore, higher encapsulation efficiency (EE%) for proteins, since larger vesicles enclose larger aqueous volumes. Publisher PDF |
| Databáze: | OpenAIRE |
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