Measuring Intracellular Viscosity in Conditions of Hypergravity
| Autor: | Jack J. W. A. van Loon, Markéta Kubánková, Ismael López-Duarte, Emma M. Woodcock, Marina K. Kuimova, Nicholas J. Brooks, Paul Girvan, Julia Eckert |
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| Přispěvatelé: | Maxillofacial Surgery (VUmc), Oral and Maxillofacial Surgery / Oral Pathology, Amsterdam Movement Sciences - Restoration and Development, VU University medical center, Engineering & Physical Science Research Council (EPSRC) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Boron Compounds
Endothelium Intracellular Space MEMBRANE VISCOSITY Biophysics CYTOSKELETON PRESSURE Mice 03 medical and health sciences Viscosity 0302 clinical medicine GRAVITY Human Umbilical Vein Endothelial Cells medicine Animals Humans ADAPTATION Cytoskeleton 030304 developmental biology FLUIDITY 0303 health sciences Hypergravity Science & Technology 02 Physical Sciences SHEAR-STRESS Chemistry 3T3 Cells Articles 06 Biological Sciences LIVE CELLS Biomechanical Phenomena MODEL medicine.anatomical_structure Membrane ENDOTHELIUM Cell culture Human umbilical vein endothelial cell 03 Chemical Sciences Life Sciences & Biomedicine 030217 neurology & neurosurgery Intracellular Gravitation |
| Zdroj: | Biophysical Journal, 116(10), 1984-1993. Biophysical Society Woodcock, E M, Girvan, P, Eckert, J, Lopez-Duarte, I, Kubánková, M, van Loon, J J W A, Brooks, N J & Kuimova, M K 2019, ' Measuring intracellular viscosity in conditions of hypergravity ', Biophysical Journal, vol. 116, no. 10, pp. 1984-1993 . https://doi.org/10.1016/j.bpj.2019.03.038 Biophys J Woodcock, E M, Girvan, P, Eckert, J, Lopez-Duarte, I, Kubánková, M, van Loon, J J W A, Brooks, N J & Kuimova, M K 2019, ' Measuring Intracellular Viscosity in Conditions of Hypergravity ', Biophysical Journal, vol. 116, no. 10, pp. 1984-1993 . https://doi.org/10.1016/j.bpj.2019.03.038 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2019.03.038 |
| Popis: | Gravity-sensitive cellular responses are regularly observed in both specialized and nonspecialized cells. One potential mechanism for this sensitivity is a changing viscosity of the intracellular organelles. Here, we report a novel, to our knowledge, viscosity-sensitive molecular rotor based on mesosubstituted boron-dipyrrin used to investigate the response of viscosity of cellular membranes to hypergravity conditions created at the large diameter centrifuge at the European Space Agency Technology Centre. Mouse osteoblastic (MC3T3-E1) and endothelial (human umbilical vein endothelial cell) cell lines were tested, and an increase in viscosity was found with increasing hypergravity loading. This response is thought to be primarily biologically driven, with the potential for a small, instantaneous physical mechanism also contributing to the observed effect. This work provides the first, to our knowledge, quantitative data for cellular viscosity changes under hypergravity, up to 15 × g. |
| Databáze: | OpenAIRE |
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