mlt Mutation in the polyomavirus genome impairing a function of the middle T protein
| Autor: | M Bastin, Celine Gelinas, S. Masse |
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| Rok vydání: | 1984 |
| Předmět: |
Antigens
Polyomavirus Transforming Immunology Mutant Biology medicine.disease_cause Microbiology Genome Viral Proteins chemistry.chemical_compound In vivo Cricetinae Virology medicine Animals Coding region Nucleotide Amino Acid Sequence chemistry.chemical_classification Genetics Mutation Base Sequence Oncogenes Cell Transformation Viral Molecular biology Cell Transformation Neoplastic chemistry Insect Science DNA Viral Polyomavirus Function (biology) DNA Research Article |
| Zdroj: | Journal of Virology. 51:242-246 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | The DNA from polyomavirus mlt mutant P155 transforms cells in culture as efficiently as wild-type DNA but has a much lower tumorigenic potential when injected into newborn rodents. The mutant has a 12-base-pair deletion between nucleotides 1347 and 1360, i.e., in a region which encodes parts of the middle and large T antigens (G elinas et al., J. Virol. 43:1072-1081, 1982). To determine which of the two viral gene functions was affected by the mutation, we transferred the latter into a modified polyomavirus genome encoding exclusively the middle T protein. Our results show that the P155 mutation alters a function of the polyomavirus middle T protein required for the induction of the tumorigenic process in vivo. Beside the 12-base-pair deletion at 96.3 map units, there is no other alteration in the coding sequence of P155 middle T with respect to that of P16, the wild-type parental strain. We conclude, therefore, that the deletion is the lesion affecting the tumorigenic potential of mutant P155 . |
| Databáze: | OpenAIRE |
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