Evaluation of Tc-99 m Labeled Dimeric GX1 Peptides for Imaging of Colorectal Cancer Vasculature
| Autor: | Liping Yao, Minglei He, Yongzhan Nie, Hao Hu, Jing Wang, Xiaoli Hui, Ming Li, Kaichun Wu, Jipeng Yin, Jing Zhang, Bo Xin |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Biodistribution Angiogenesis Mice Nude Peptide Sensitivity and Specificity Umbilical vein Mice In vivo Cell Line Tumor medicine Animals Humans Radiology Nuclear Medicine and imaging Tissue Distribution chemistry.chemical_classification business.industry Technetium In vitro Molecular Imaging Oncology chemistry Cancer research PEGylation Molecular imaging Radiopharmaceuticals business Colorectal Neoplasms |
| Zdroj: | Molecular imaging and biology. 17(5) |
| ISSN: | 1860-2002 |
| Popis: | This study aimed to evaluate the potential of PEGylated dimeric GX1 peptide as a radiotracer for imaging of colorectal cancer vasculature in a LoVo tumor xenografted mouse model. The [99mTc]PEG-(GX1)2 peptide was synthesized and identified. Confocal immunofluorescence analysis, receptor binding assay, and competitive inhibition assay were performed to evaluate the binding specificity and the receptor binding affinity of PEG-(GX1)2 to Co-human umbilical vein endothelial cells (HUVECs). Single photon emission computed tomography imaging and biodistribution were performed to evaluate the targeting ability of PEG-(GX1)2 to colorectal cancer. The studies in vitro suggested that PEG-(GX1)2 co-localized with Factor VIII in the perinuclear cytoplasm of Co-HUVECs and bound specifically to Co-HUVECs with a high affinity. The studies in vivo demonstrated that the targeting efficacy of PEG-(GX1)2 was superior to GX1. PEGylation improved the affinity and the targeting ability of the GX1 peptide. PEG-(GX1)2 is a more promising probe for imaging of colorectal vasculature than GX1. |
| Databáze: | OpenAIRE |
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