Determination of Terpenoid Content in Pine by Organic Solvent Extraction and Fast-GC Analysis
| Autor: | Anne E. Harman-Ware, Gary F. Peter, Mark F. Davis, Robert W. Sykes |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Economics and Econometrics Energy Engineering and Power Technology lcsh:A 03 medical and health sciences chemistry.chemical_compound Organic chemistry fast-GC Oleoresin Pinene Chromatography Humulene Renewable Energy Sustainability and the Environment Caryophyllene Extraction (chemistry) Energy Research biofuels Terpenoid Hexane pyrolysis-molecular beam mass spectrometry renewable materials 030104 developmental biology Fuel Technology cell wall chemistry chemistry Camphene bioproducts lcsh:General Works biomaterials |
| Zdroj: | Frontiers in Energy Research, Vol 4 (2016) |
| ISSN: | 2296-598X |
| DOI: | 10.3389/fenrg.2016.00002 |
| Popis: | Terpenoids, naturally occurring compounds derived from isoprene units present in pine oleoresin, are a valuable source of chemicals used in solvents, fragrances, flavors, and have shown potential use as a biofuel. This paper describes a method to extract and analyze the terpenoids present in loblolly pine saplings and pine lighter wood. Various extraction solvents were tested over different times and temperatures. Samples were analyzed by pyrolysis-molecular beam mass spectrometry before and after extractions to monitor the extraction efficiency. The pyrolysis studies indicated that the optimal extraction method used a 1:1 hexane/acetone solvent system at 22°C for 1 h. Extracts from the hexane/acetone experiments were analyzed using a low thermal mass modular accelerated column heater for fast-GC/FID analysis. The most abundant terpenoids from the pine samples were quantified, using standard curves, and included the monoterpenes, α- and β-pinene, camphene, and δ-carene. Sesquiterpenes analyzed included caryophyllene, humulene, and α-bisabolene. Diterpenoid resin acids were quantified in derivatized extractions, including pimaric, isopimaric, levopimaric, palustric, dehydroabietic, abietic, and neoabietic acids. |
| Databáze: | OpenAIRE |
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