Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae)

Autor: Maxime Eeraerts, Matti Pisman, Gregor Claus, Guy Smagghe, Pieter Spanoghe
Rok vydání: 2021
Předmět:
Agriculture and Food Sciences
Insecticides
Health
Toxicology and Mutagenesis
Thiazines
0211 other engineering and technologies
02 engineering and technology
Hymenoptera
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Environmental pollution
Toxicology
Neonicotinoids
chemistry.chemical_compound
GE1-350
Lethal endpoints
media_common
Larva
biology
Longevity
General Medicine
Bees
Thiacloprid
Pollution
Solitary bee larvae
TD172-193.5
Toxicity
Pollen
Female
Megachilidae
Pesticide risk assessment
media_common.quotation_subject
Toxicity Tests
Sublethal endpoints
medicine
Animals
Pesticides
0105 earth and related environmental sciences
Life Cycle Stages
021110 strategic
defence & security studies
fungi
Public Health
Environmental and Occupational Health
Pesticide
biology.organism_classification
Environmental sciences
chemistry
Earth and Environmental Sciences
Oral test protocol
Zdroj: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Ecotoxicology and Environmental Safety, Vol 215, Iss, Pp 112143-(2021)
ISSN: 0147-6513
1090-2414
DOI: 10.1016/j.ecoenv.2021.112143
Popis: Risk assessment of pesticides involves ecotoxicological testing. In case pesticide exposure to bees is likely, toxicity tests are performed with honey bees (Apis mellifera), with a tiered approach, for which validated and internationally accepted test protocols exist. However, concerns have grown regarding the protection of non-Apis bees [bumble bees (Bombus spp.), solitary and stingless bees], given their different life cycles and therefore distinct exposure routes. Larvae of solitary bees of the genus Osmia feed on unprocessed pollen during development, yet no toxicity test protocol is internationally accepted or validated to assess the impact of pesticide exposure during this stage of their life cycle. Therefore, the purpose of this study is to further validate a test protocol with two solitary bee species (O. cornuta and O. bicornis) to assess lethal and sublethal effects of pesticide exposure on larval development. Larvae were exposed to thiacloprid (neonicotinoid insecticide) mixed in a new, artificial pollen provision. Both lethal (developmental and winter mortality) and sublethal endpoints (larval development time, pollen provision consumption, cocoon weight, emergence time and adult longevity) were recorded. Effects of lower, more environmentally realistic doses were only reflected in sublethal endpoints. In both bee species, thiacloprid treatment was associated with increased developmental mortality and larval development time, and decreased pollen provision consumption and cocoon weight. The test protocol proved valid and robust and showed that for higher doses of thiacloprid the acute endpoint (larval mortality) is sufficient. In addition, new insights needed to develop a standardized test protocol were acquired, such as testing of a positive control for the first time and selection of male and female individuals at egg level.
Databáze: OpenAIRE
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