Identification and Characterization of Proteins Involved in Nuclear Organization Using Drosophila GFP Protein Trap Lines
| Autor: | Kyler Griffin, Felicia Graves, Deaundra Jackson, Elyas Munye, Kim Yen Thi Vu, Karen Wu, Elisha Pittman, Derica Laramore, Keith Jones, Bramwel Kithuka, Anwar Shagarabi, B. V. Gurudatta, Julia Davis, Britney McCrary, Nikkita Roberts, De’Warren Rose, Teanndras Miller, Kai Sung, Eduardo A. Torre, Alyssia Clore, Mark Araujo, Brian Mott, Victor G. Corces, Marquita Kilgore, Margaret Rohrbaugh, Maza R. Tchedou, Thuy Le, Gabriel Duffy, Tayler Stallworth, Jarvis Veira, Krystal Lunsford, Sharonta Johnson, Jingping Yang, Ashley M. Wood, Alex Rowland, Elizabeth Sung, Stuart Cushing, Kendall Jamison, Khali Jones, William Wagstaff, Naomi Takenaka, Bakhtawar Mazhar, Dhaujee Kelley, Denis Jaimes, Alex Mullins, Nicole Stroud, Sheila Okoorie, Joy Mitchell, Joanne Kim, Celethia Moreland, Jamela Smith, Edward Ramos, Muhammad M. Mazhar, Gilbert Acosta, Brian G. Jones |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Ubiquitin-Protein Ligases DNA transcription Green Fluorescent Proteins lcsh:Medicine Biochemistry Models Biological Green fluorescent protein Model Organisms Molecular cell biology Nucleic Acids medicine Animals Drosophila Proteins Protein Interaction Maps lcsh:Science Nucleoplasmins Biology Polytene Chromosomes Cell Nucleus Multidisciplinary Polytene chromosome Nuclear Lamina biology Chromosome Biology Drosophila Melanogaster lcsh:R Proteins Cell Differentiation Animal Models biology.organism_classification Lamin Type A Molecular biology Diploidy Cellular Structures Chromatin Cell nucleus medicine.anatomical_structure Germ Cells Protein Classes Nuclear lamina lcsh:Q Gene expression Drosophila melanogaster Drosophila Protein Lamin Heat-Shock Response Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 1, p e53091 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Background Strains from a collection of Drosophila GFP protein trap lines express GFP in the normal tissues where the endogenous protein is present. This collection can be used to screen for proteins distributed in the nucleus in a non-uniform pattern. Methodology/Principal Findings We analyzed four lines that show peripheral or punctate nuclear staining. One of these lines affects an uncharacterized gene named CG11138. The CG11138 protein shows a punctate distribution in the nuclear periphery similar to that of Drosophila insulator proteins but does not co-localize with known insulators. Interestingly, mutations in Lamin proteins result in alterations in CG11138 localization, suggesting that this protein may be a novel component of the nuclear lamina. A second line affects the Decondensation factor 31 (Df31) gene, which encodes a protein with a unique nuclear distribution that appears to segment the nucleus into four different compartments. The X-chromosome of males is confined to one of these compartments. We also find that Drosophila Nucleoplasmin (dNlp) is present in regions of active transcription. Heat shock leads to loss of dNlp from previously transcribed regions of polytene chromosome without redistribution to the heat shock genes. Analysis of Stonewall (Stwl), a protein previously found to be necessary for the maintenance of germline stem cells, shows that Stwl is present in a punctate pattern in the nucleus that partially overlaps with that of known insulator proteins. Finally we show that Stwl, dNlp, and Df31 form part of a highly interactive network. The properties of other components of this network may help understand the role of these proteins in nuclear biology. Conclusions/Significance These results establish screening of GFP protein trap alleles as a strategy to identify factors with novel cellular functions. Information gained from the analysis of CG11138 Stwl, dNlp, and Df31 sets the stage for future studies of these proteins. |
| Databáze: | OpenAIRE |
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