Maitake (D Fraction) Mushroom Extract Induces Apoptosis in Breast Cancer Cells by BAK-1 Gene Activation
| Autor: | Eliana Noelia Alonso, Manuela Meireles, Diego Javier Obiol, Ana Rocha, Gabriela A. Balogh, Gisela Joos, Raquel Soares, Ana Pirraco |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Transcriptional Activation
CIENCIAS MÉDICAS Y DE LA SALUD Maitake Medicine (miscellaneous) Breast Neoplasms Apoptosis Pharmacology Mushroom Biological Factors Cell Line Tumor Breast Cancer Humans Viability assay Bak-1 Incubation Nutrition and Dietetics Deoxyuridine Triphosphate biology Cytochrome c Cytochrome C Bioquímica y Biología Molecular Gene Expression Regulation Neoplastic Medicina Básica bcl-2 Homologous Antagonist-Killer Protein Biochemistry Cell culture Cancer cell biology.protein Female Grifola |
| DOI: | 10.1089/jmf.2010.0095 |
| Popis: | For many years mushrooms have been used empirically in traditional medicine to treat several diseases. Study of the maitake mushroom, with its immunomodulatory and antitumoral properties, has led to the isolation of several bioactive compounds. One of these, D fraction, is known to reduce tumor cell viability. This study examined the effect of isolated D fraction on viability and apoptosis of human breast cancer cells (MCF7). These cells were treated with maitake (D fraction) extract at 18μg/mL, 36μg/mL, 91μg/mL, 183μg/mL, or 367μg/mL or were left untreated (control) for 24 hours. MCF7 incubation with the maitake extract resulted in decreased cell viability [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium assay] in a dose-dependent manner. Apoptosis was statistically significantly increased in a dose-dependent manner at every concentration tested (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assay). Upon incubation with D fraction, a microarray assay revealed upregulation of BAK-1 and cytochrome c transcripts, 2 proteins directly involved in the apoptotic pathway. Reverse transcriptase polymerase chain reaction studies confirmed these findings; BAK-1 was one of most overexpressed gene, as observed by microarray assay. These findings confirm the apoptotic effect of maitake D fraction in breast cancer cells and further highlight the involvement of cytochrome c release to the cytoplasm. Cytoplasmic release of cytochrome c, another player in the apoptotic pathway, was also increased after incubation with D fraction in a dose-dependent manner. This finding indicates that the effect of this compound involves mitochondrial dysfunction. The identification of the molecular mechanisms by which D fraction exerts its effects is crucial for the development of preventive and therapeutic strategies for cancer. Fil: Soares, Raquel. Universidad de Porto; Portugal Fil: Meireles, Manuela. Universidad de Porto; Portugal Fil: Rocha, Ana. Universidad de Porto; Portugal Fil: Pirraco, Ana. Universidad de Porto; Portugal Fil: Obiol, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Alonso, Eliana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Joos, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Balogh, Gabriela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina |
| Databáze: | OpenAIRE |
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