The impact of c-kit and ki-67 expression on patients prognosis in advanced ovarian serous carcinoma
Autor: | Ibrahim Khalifeh, W. Dwayne Lawrence, Adnan R. Munkarah, Rouba Ali-Fehmi, Robert T. Morris, Veronica Schimp |
---|---|
Rok vydání: | 2005 |
Předmět: |
Adult
Pathology medicine.medical_specialty Proliferation index Serous carcinoma Gene Expression Pathology and Forensic Medicine Statistical significance medicine Biomarkers Tumor Humans Survival analysis Aged Proportional Hazards Models Retrospective Studies Aged 80 and over Ovarian Neoplasms biology Proportional hazards model Obstetrics and Gynecology Odds ratio Middle Aged medicine.disease Prognosis Immunohistochemistry Survival Analysis Confidence interval Cystadenocarcinoma Serous Proto-Oncogene Proteins c-kit Ki-67 Antigen Proto-Oncogene Proteins c-bcl-2 Ki-67 biology.protein Female Tumor Suppressor Protein p53 |
Zdroj: | International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists. 24(3) |
ISSN: | 0277-1691 |
Popis: | The transmembrane-tyrosine-kinase receptor, c-kit, is involved in cell differentiation and has been found to be expressed in normal human cell types and solid tumors. This study was designed to investigate the effects of c-kit expression on: 1) tumor proliferation and apoptosis, and 2) survival in patients with high-grade advanced stage ovarian serous carcinoma (OSC). We identified 118 patients with high-grade advanced stage OSC from our files. Clinical data, including demographics and overall survival, were collected. Immunohistochemical panel consisting of c-kit, ki-67, p53, and bcl-2 was performed. C-kit was categorized as positive if any cytoplasmic or membranous staining pattern was identified. Correlation between c-kit expression and the other markers was performed. Survival analysis was performed using COX proportional hazards regression and Kaplan-Meier test. Of 118 cases, 25 (21.2%) expressed c-kit. Of 93 c-kit-negative tumors, 87.1% had a high proliferation index. High p53 and bcl-2 expression was identified in 96 (81.4%) and 59 (50%) cases respectively. No significant statistical correlation was identified between c-kit and apoptosis markers. Tumors lacking c-kit expression showed a trend toward having high proliferation index, but this did not achieve statistical significance (p = 0.07). Of the seven variables included in the multivariate survival analysis, only c-kit (odds ratio, 2.12; 95% confidence interval, 08-4.17; p = 0.02) and ki-67 (odds ratio, 1.9; 95% confidence interval, 1.1-3.1; p = 0.03) showed an independent statistically significant impact on survival. High-grade advanced stage OSC lacking c-kit expression correlates with poor outcome. Interestingly, cases lacking c-kit expression also showed a trend to have high proliferation index. |
Databáze: | OpenAIRE |
Externí odkaz: |