The correlation of RANK, RANKL and TNFalpha expression with bone loss volume and polyethylene wear debris around hip implants
| Autor: | Helen Lucas, Donald W. Howie, Kush R. Shrestha, David R. Haynes, C.A. Holding, Anak A S S K Dharmapatni, Gerald J. Atkins, Stuart A. Callary, Roumen Stamenkov, Susan D. Neale, David M. Findlay |
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| Rok vydání: | 2006 |
| Předmět: |
musculoskeletal diseases
Pathology medicine.medical_specialty Necrosis Materials science Osteolysis medicine.medical_treatment Arthroplasty Replacement Hip Biophysics Osteoclasts Receptors Cytoplasmic and Nuclear Bioengineering Osteoarthritis Receptors Tumor Necrosis Factor Bone remodeling Biomaterials Osteoclast medicine Humans Bone Resorption Glycoproteins Hip Membrane Glycoproteins biology Receptor Activator of Nuclear Factor-kappa B Tumor Necrosis Factor-alpha RANK Ligand Osteoprotegerin medicine.disease Immunohistochemistry medicine.anatomical_structure Cytokine Mechanics of Materials RANKL Ceramics and Composites biology.protein Hip Prosthesis medicine.symptom Polyethylenes Carrier Proteins Tomography X-Ray Computed |
| Zdroj: | Biomaterials. 27(30) |
| ISSN: | 0142-9612 |
| Popis: | This study investigates receptor activator NF-kB (RANK), RANK ligand (RANKL) and tumour necrosis factor (TNFa), key factors regulating bone turnover, present in the tissues near peri-prosthetic osteolysis. Tissue was obtained from zones of peri-prosthetic osteolysis from 11 patients undergoing revision of total hip prostheses, analysed preoperatively by high-resolution spiral multislice CT using a metal artefact suppression protocol. Synovial tissue from 10 patients with osteoarthritis undergoing primary hip replacement was used as control tissue. Immunohistochemical analysis of formalin fixed tissue sections demonstrated that RANK, RANKL and TNFa were strongly expressed by large multinucleated cells containing polyethylene wear debris in revision tissues. Control tissue stained weakly for RANK, RANKL and TNFa. A strong statistical correlation (po0:02) was found between the five parameters, volume of bone loss, polyethylene wear debris, RANK, RANKL and TNFa expression. Importantly, in vitro studies revealed that RANKL and TNFa synergise to increase the volume of bone resorbed, by more than seven fold, when compared to the effect of either cytokine treatment alone. This suggests that the interaction of TNFa and RANKL promotes osteoclast activity associated with polyethylene wear and therapies targeting TNF activity may be useful to treat peri-implant osteolysis. r 2006 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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