Immunofluorescence versus xTAG multiplex PCR for the detection of respiratory picornavirus infections in children
| Autor: | Ann Lea Kraemer, Maria Teresa Barbani, M. Gorgievski-Hrisoho, Christoph Aebi, Nicolas Regamey, Christina Schindera |
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| Rok vydání: | 2009 |
| Předmět: |
Picornavirus
viruses Immunofluorescence Fluorescent Antibody Technique Picornaviridae ADV Adenovirus INB Influenzavirus B Polymerase Chain Reaction Sensitivity and Specificity Article Enteroviruses Human metapneumovirus Nasopharynx Virology Multiplex polymerase chain reaction medicine Humans Child HMPV Human metapneumovirus Respiratory Tract Infections Children IFA Influenzavirus A Picornaviridae Infections biology Influenzavirus B IF Immunofluorescence Respiratory disease Infant Newborn Respiratory infection Infant biology.organism_classification medicine.disease NPA Nasopharyngeal aspirate Pneumonia Infectious Diseases PCR Child Preschool Rhinoviruses Immunology Bronchitis RSV Respiratory syncytial virus PIV Parainfluenza virus Respiratory picornaviruses PCR Polymerase chain reaction |
| Zdroj: | Journal of Clinical Virology |
| ISSN: | 1873-5967 |
| Popis: | Background Polymerase chain reaction (PCR) is a sensitive tool for detection of respiratory picornaviruses. However, the clinical relevance of picornavirus detection by PCR is unclear. Immunofluorescence (IF), widely used to detect other respiratory viruses, has recently been introduced as a promising detection method for respiratory picornaviruses. Objectives To compare the clinical manifestations of respiratory picornavirus infections detected by IF with those of respiratory picornavirus infections detected by xTAG multiplex PCR in hospitalized children. Study design During a 1-year period, nasopharyngeal aspirates (NPA) from all children hospitalized due to an acute respiratory infection were prospectively analyzed by IF. All respiratory picornavirus positive IF samples and 100 IF negative samples were further tested with xTAG multiplex PCR. After exclusion of children with co-morbidities and viral co-infections, monoinfections with respiratory picornaviruses were detected in 108 NPA of 108 otherwise healthy children by IF and/or PCR. We compared group 1 children (IF and PCR positive, n = 84) with group 2 children (IF negative and PCR positive, n = 24) with regard to clinical manifestations of the infection. Results Wheezy bronchitis was diagnosed more often in group 1 than in group 2 (71% vs. 46%, p = 0.028). In contrast, group 2 patients were diagnosed more frequently with pneumonia (17% vs. 6%, p = 0.014) accompanied by higher levels of C-reactive protein (46 mg/l vs. 11 mg/l, p = 0.009). Conclusions Picornavirus detection by IF in children with acute respiratory infection is associated with the clinical presentation of wheezy bronchitis. The finding of a more frequent diagnosis of pneumonia in picornavirus PCR positive but IF negative children warrants further investigation. |
| Databáze: | OpenAIRE |
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