Allograft inflammatory factor-1 stimulates chemokine production and induces chemotaxis in human peripheral blood mononuclear cells
| Autor: | Katsura Mizushima, Hiroshi Obayashi, Ryo Oda, Hiroyoshi Fujiwara, Masataka Kohno, Masahide Hamaguchi, Masatoshi Kadoya, Takahiro Seno, Aihiro Yamamoto, Yutaka Kawahito, Mitsuhiro Ohta |
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| Rok vydání: | 2014 |
| Předmět: |
Chemokine
CD14 Lipopolysaccharide Receptors Biophysics CCL1 Biology CCL7 Biochemistry Peripheral blood mononuclear cell Monocytes Humans CCL17 RNA Messenger education Molecular Biology Chemokine CCL3 education.field_of_study Interleukin-6 Calcium-Binding Proteins Microfilament Proteins hemic and immune systems Chemotaxis Cell Biology Molecular biology Recombinant Proteins Up-Regulation DNA-Binding Proteins Chemotaxis Leukocyte Chemokines CC Immunology Allograft inflammatory factor 1 biology.protein Transcriptome |
| Zdroj: | Biochemical and Biophysical Research Communications. 448:287-291 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2014.04.106 |
| Popis: | Allograft inflammatory factor-1 (AIF-1) is expressed by macrophages, fibroblasts, endothelial cells and smooth muscle cells in immune-inflammatory disorders such as systemic sclerosis, rheumatoid arthritis and several vasculopathies. However, its molecular function is not fully understood. In this study, we examined gene expression profiles and induction of chemokines in monocytes treated with recombinant human AIF (rhAIF-1). Using the high-density oligonucleotide microarray technique, we compared mRNA expression profiles of rhAIF-1-stimulated CD14(+) peripheral blood mononuclear cells (CD14(+) PBMCs) derived from healthy volunteers. We demonstrated upregulation of genes for several CC chemokines such as CCL1, CCL2, CCL3, CCL7, and CCL20. Next, using ELISAs, we confirmed that rhAIF-1 promoted the secretion of CCL3/MIP-1α and IL-6 by CD14(+) PBMCs, whereas only small amounts of CCL1, CCL2/MCP-1, CCL7/MCP-3 and CCL20/MIP-3α were secreted. Conditioned media from rhAIF-1stimulated CD14(+) PBMCs resulted in migration of PBMCs. These findings suggest that AIF-1, which induced chemokines and enhanced chemotaxis of monocytes, may represent a molecular target for the therapy of immune-inflammatory disorders. |
| Databáze: | OpenAIRE |
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