The hUPF1-NMD factor controls the cellular transcript levels of different genes of complex I of the respiratory chain
| Autor: | Anna Maria Sardanelli, Sergio Papa, Damiano Panelli, Alessandro Stella, Francesca Paola Lorusso, Raffaella Trentadue |
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| Rok vydání: | 2012 |
| Předmět: |
Nonsense-mediated decay
Respiratory chain Biology Biochemistry Transcriptome Electron Transport Complex IV Humans Transcription factor Gene Genetics Gene knockdown Electron Transport Complex I Reverse Transcriptase Polymerase Chain Reaction Alternative splicing RNA-Binding Proteins General Medicine Nonsense Mediated mRNA Decay Isoenzymes Alternative Splicing Codon Nonsense Trans-Activators RNA Interference RNA Helicases HeLa Cells Transcription Factors |
| Zdroj: | Biochimie. 94(12) |
| ISSN: | 1638-6183 |
| Popis: | In this study the impact of hUPF1 and hUPF2 knockdown on alternative splicing (AS) isoforms of different genes encoding subunits of respiratory chain complex I and complex IV is described. As expected, loss of both hUPF1 and hUPF2 led to impairment of nonsense-mediated mRNA decay (NMD) and accumulation of PTC-containing NMD substrates generated by both complex I and complex IV genes. The levels of some complex I splice variants, which did not contain PTC as well as the level of some complex I canonical transcripts were, however, affected only by hUPF1 knockdown. This finding confirms that NMD plays a role in the maintenance of the transcriptome integrity and reveals a specific impact of hUPF1 on the regulation of complex I genes. |
| Databáze: | OpenAIRE |
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