Microglial activation in the right amygdala-entorhinal-hippocampal complex is associated with preserved spatial learning in App mice
| Autor: | Florian Eckenweber, Michael Willem, Simon Lindner, Karin Wind, Barbara von Ungern-Sternberg, Sabina Tahirovic, Tanja Blume, Christian Sacher, Peter Bartenstein, Axel Rominger, Michael Ewers, Gloria Biechele, Leonie Beyer, Jochen Herms, Matthias Brendel, Nicolai Franzmeier, Paul Cumming, Franz-Josef Gildehaus, Benedikt Zott |
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| Rok vydání: | 2020 |
| Předmět: |
Male
microglia Hippocampus Morris water navigation task genetics [Alzheimer Disease] metabolism [Hippocampus] metabolism [Microglia] Hippocampal formation Amyloid beta-Protein Precursor Mice 0302 clinical medicine Piriform cortex Entorhinal Cortex metabolism [Amygdala] 610 Medicine & health biosynthesis [Receptors GABA] β-amyloid spatial learning methods [Positron-Emission Tomography] 05 social sciences biosynthesis [Amyloid beta-Protein Precursor] Amygdala ddc medicine.anatomical_structure Neurology genetics [Amyloid beta-Protein Precursor] Hypothalamus Female Microglia metabolism [Alzheimer Disease] asymmetry regional heterogeneity Cognitive Neuroscience Spatial Learning Mice Transgenic Biology 050105 experimental psychology Lateralization of brain function lcsh:RC321-571 03 medical and health sciences Receptors GABA Alzheimer Disease Translocator protein medicine Animals Humans 0501 psychology and cognitive sciences ddc:610 lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry physiology [Spatial Learning] AppNL-G-F genetics [Receptors GABA] Mice Inbred C57BL App(NL-G-F) Positron-Emission Tomography biology.protein metabolism [Entorhinal Cortex] Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | NeuroImage, Vol 230, Iss, Pp 117707-(2021) Biechele, Gloria; Wind, Karin; Blume, Tanja; Sacher, Christian; Beyer, Leonie; Eckenweber, Florian; Franzmeier, Nicolai; Ewers, Michael; Zott, Benedikt; Lindner, Simon; Gildehaus, Franz-Josef; von Ungern-Sternberg, Barbara; Tahirovic, Sabina; Willem, Michael; Bartenstein, Peter; Cumming, Paul; Rominger, Axel; Herms, Jochen; Brendel, Matthias (2021). Microglial Activation in the Right Amygdala-Entorhinal-Hippocampal Complex is Associated with Preserved Spatial Learning in AppNL-G-F mice. NeuroImage, 230, p. 117707. Elsevier 10.1016/j.neuroimage.2020.117707 NeuroImage 230, 117707-(2021). doi:10.1016/j.neuroimage.2020.117707 |
| DOI: | 10.1016/j.neuroimage.2020.117707 |
| Popis: | Background In Alzheimer`s disease (AD), regional heterogeneity of β-amyloid burden and microglial activation of individual patients is a well-known phenomenon. Recently, we described a high incidence of inter-individual regional heterogeneity in terms of asymmetry of plaque burden and microglial activation in β-amyloid mouse models of AD as assessed by positron-emission-tomography (PET). We now investigate the regional associations between amyloid plaque burden, microglial activation, and impaired spatial learning performance in transgenic mice in vivo. Methods In 30 AppNL-G-F mice (15 female, 15 male) we acquired cross-sectional 18 kDa translocator protein (TSPO-PET, 18F-GE-180) and β-amyloid-PET (18F-florbetaben) scans at ten months of age. Control data were obtained from age- and sex-matched C57BI/6 wild-type mice. We assessed spatial learning (i.e. Morris water maze) within two weeks of PET scanning and correlated the principal component of spatial learning performance scores with voxel-wise β-amyloid and TSPO tracer uptake maps in AppNL-G-F mice, controlled for age and sex. In order to assess the effects of hemispheric asymmetry, we also analyzed correlations of spatial learning performance with tracer uptake in bilateral regions of interest for frontal cortex, entorhinal/piriform cortex, amygdala, and hippocampus, using a regression model. We tested the correlation between regional asymmetry of PET biomarkers with individual spatial learning performance. Results Voxel-wise analyses in AppNL-G-F mice revealed that higher TSPO-PET signal in the amygdala, entorhinal and piriform cortices, the hippocampus and the hypothalamus correlated with spatial learning performance. Region-based analysis showed significant correlations between TSPO expression in the right entorhinal/piriform cortex and the right amygdala and spatial learning performance, whereas there were no such correlations in the left hemisphere. Right lateralized TSPO expression in the amygdala predicted better performance in the Morris water maze (β = -0.470, p = 0.013), irrespective of the global microglial activation and amyloid level. Region-based results for amyloid-PET showed no significant associations with spatial learning. Conclusion Elevated microglial activation in the right amygdala-entorhinal-hippocampal complex of AppNL-G-F mice is associated with better spatial learning. Our findings support a protective role of microglia on cognitive function when they highly express TSPO in specific brain regions involved in spatial memory. |
| Databáze: | OpenAIRE |
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