The hemibiotrophic cacao pathogen Moniliophthora perniciosa depends on a mitochondrial alternative oxidase for biotrophic development
| Autor: | Odalys Garcia, Ione Salgado, Paulo José Pereira Lima Teixeira, Osvaldo Reis, Gonçalo A.G. Pereira, Daniela P. T. Thomazella, Isabella Macedo Toni, Johana Rincones, Halley Caixeta Oliveira, Elzira Elisabeth Saviani, Lyndel W. Meinhardt |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Alternative oxidase
Hypha Physiology Respiratory chain Gene Expression Plant Science Fungus Trypanosoma brucei Nitric Oxide Moniliophthora perniciosa Microbiology Mitochondrial Proteins hemibiotrophic Salicylamides Gene Pathogen nitric oxide (NO) Plant Diseases Plant Proteins cacao (Theobroma cacao) Cacao biology Mycelium Research biology.organism_classification Strobilurins Mitochondria Up-Regulation Pyrimidines phase transition Host-Pathogen Interactions alternative oxidase (AOX) Methacrylates Agaricales Oxidoreductases witches’ broom disease (WBD) |
| Zdroj: | The New Phytologist |
| ISSN: | 1469-8137 0028-646X |
| Popis: | The tropical pathogen Moniliophthora perniciosa causes witches' broom disease in cacao. As a hemibiotrophic fungus, it initially colonizes the living host tissues (biotrophic phase), and later grows over the dead plant (necrotrophic phase). Little is known about the mechanisms that promote these distinct fungal phases or mediate the transition between them. An alternative oxidase gene (Mp-aox) was identified in the M. perniciosa genome and its expression was analyzed througout the fungal life cycle. In addition, the effects of inhibitors of the cytochrome-dependent respiratory chain (CRC) and alternative oxidase (AOX) were evaluated on the in vitro development of M. perniciosa. Larger numbers of Mp-aox transcripts were observed in the biotrophic hyphae, which accordingly showed elevated sensitivity to AOX inhibitors. More importantly, the inhibition of CRC prevented the transition from the biotrophic to the necrotrophic phase, and the combined use of a CRC and AOX inhibitor completely halted fungal growth. On the basis of these results, a novel mechanism is presented in which AOX plays a role in the biotrophic development of M. perniciosa and regulates the transition to its necrotrophic stage. Strikingly, this model correlates well with the infection strategy of animal pathogens, particularly Trypanosoma brucei, which uses AOX as a strategy for pathogenicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text