Quantitative morphological changes in endocrine pancreas of rats with spontaneous diabetes mellitus
| Autor: | M.C. Semino, Juan José Gagliardino, César L.A. Gómez Dumm |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
medicine.medical_specialty geography geography.geographical_feature_category Endoplasmic reticulum Pancreatic islets Rats Inbred Strains Biology medicine.disease Islet Diabetes Mellitus Experimental Rats Pathogenesis Islets of Langerhans Microscopy Electron medicine.anatomical_structure Endocrinology Fibrosis Diabetes mellitus Internal medicine medicine Endocrine system Animals cardiovascular diseases Pancreas |
| Zdroj: | Virchows Archiv. B, Cell pathology including molecular pathology. 57(6) |
| ISSN: | 0340-6075 |
| Popis: | The present study describes the cytopathology of the pancreatic islets in 18-old male eSS rats with spontaneous diabetes mellitus as compared to aged-matched normal animals. Light-microscopic immunocytochemical and morphometric techniques were used to study islet-cell populations, while quantitative methods were employed specifically for the analysis of B-cell ultrastructure. The diabetic rats showed disruption of the islet structure and fibrosis in the stroma. The volume density (Vvi) of endocrine tissue and the Vvi and percentage of B cells were diminished, whereas the Vvi of exocrine tissue and the Vvi and percentage of D cells were increased. The number of medium and large islets as well as their mean volume (micron3) decreased in these animals. Pancreatic B cells from eSS rats showed an increase in the Vvi of endoplasmic reticulum, immature secretory granules and lysosomes. Conversely, the Vvi of total secretory granules and microtubules appeared diminished. The current observations contribute to our understanding of this useful animal model of diabetes mellitus, in the attempt to clarify the pathogenesis of the disease. |
| Databáze: | OpenAIRE |
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