The KbvR Regulator Contributes to Capsule Production, Outer Membrane Protein Biosynthesis, Antiphagocytosis, and Virulence in Klebsiella pneumoniae
| Autor: | Fusheng Zhang, Li Xu, Jie Yuan, Jing Yang, Meng Wang, Hui Wang, Moran Li, Jingjie Wang, Yujiao Tian, Bei Li |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Klebsiella pneumoniae Phagocytosis 030106 microbiology Immunology Regulator Virulence Microbiology Transcriptome 03 medical and health sciences Immune system Bacterial Proteins Animals Humans Bacterial Capsules Innate immune system biology Polysaccharides Bacterial Gene Expression Regulation Bacterial biology.organism_classification Molecular Pathogenesis Immunity Innate Klebsiella Infections 030104 developmental biology Infectious Diseases Host-Pathogen Interactions Mutation Parasitology Bacterial outer membrane Bacterial Outer Membrane Proteins |
| Zdroj: | Infect Immun |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Klebsiella pneumoniae is an opportunistic pathogen that mostly affects patients with weakened immune systems, but a few serotypes (especially K1 and K2) are highly invasive and result in systemic infection in healthy persons. The ability to evade and survive the components of the innate immune system is critical in infection. To investigate the role and mechanism of transcription regulator KP1_RS12260 (KbvR) in virulence and defense against the innate immune response, kbvR deletion mutant and complement strains were constructed. The in vivo animal infection assay and in vitro antiphagocytosis assay demonstrate K. pneumoniae KbvR is an important regulator that contributes to virulence and the defense against phagocytosis of macrophages. The transcriptome analysis and phenotype experiments demonstrated that deletion of kbvR decreased production of capsular polysaccharide (CPS) and biosynthesis of partly outer membrane proteins (OMPs). The findings suggest that KbvR is a global regulator that confers pathoadaptive phenotypes, which provide several implications for improving our understanding of the pathogenesis of K. pneumoniae. |
| Databáze: | OpenAIRE |
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