ITGA4 gene methylation status in chronic lymphocytic leukemia
| Autor: | Hanaa R.M. Attia, Randa A. Osman, Shereen H Abd El-Aziz, Mohamed Emam Sobeih, Naglaa M. Hassan, Amany H. Abdelrahman, Heba A. Hagag, Marianne E. Yassa, Mona Hamed Ibrahim, Iman I. Salama |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Chronic lymphocytic leukemia Biology cytogenetics 03 medical and health sciences 0302 clinical medicine hemic and lymphatic diseases Methylation analysis Gene expression medicine methylation analysis beta 2 microglobulin Beta-2 microglobulin Cytogenetics ITGA4 gene expression Methylation medicine.disease pyrosequencing 030104 developmental biology 030220 oncology & carcinogenesis DNA methylation Cancer research chronic lymphocytic leukemia Pyrosequencing prognostic markers Research Article Biotechnology |
| Zdroj: | Future Science OA |
| ISSN: | 2056-5623 |
| Popis: | Background: We aimed to investigate ITGA4 gene expression pattern and to explore its methylation heterogeneity in chronic lymphocytic leukemia (CLL). Patients & methods: Eighty one CLL patients and 75 healthy subjects were enrolled and prognostic evaluation of patients was assessed. ITGA4 q-realtime PCR was performed using Applied Biosystems, TaqMan gene expression assay. ITGA4 gene-specific CpG methylation was investigated in real time using pyrosequencing technology. Results: ITGA4 was differentially expressed in CLL patients. The CpG sites-1, 2 and 3 showed significantly higher mean levels than healthy controls (p = Lay abstract Chronic lymphocytic leukemia (CLL) is a clonal lymphoproliferative disorder that is characterized by heterogeneous presentation. The ITGA4 gene (CD49d) encodes a member of the integrin alpha chain family of proteins. With this study we aimed to investigate the ITGA4 gene expression by q-realtime PCR and to explore its methylation heterogeneity using pyrosequencing technology in CLL. We found that the ITGA4 gene was differentially expressed in CLL patients. A significant association between CpG site-1 and CLL has been detected using age-adjusted logistic regression analysis. Hypermethylation at ITGA4 gene CpG sites (1,2,3) is a characteristic feature in CLL when compared with healthy controls. |
| Databáze: | OpenAIRE |
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