Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells
| Autor: | M. J. Roberti, Elizabeth A. Jares-Erijman, Thomas M. Jovin, Pietrasanta Li, Menéndez G, Marcos Morgan |
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| Rok vydání: | 2009 |
| Předmět: |
Amyloid
Chemistry Nanoparticle General Chemistry Biosensing Techniques Microscopy Atomic Force Biochemistry Molecular biology Catalysis In vitro nervous system diseases Colloid and Surface Chemistry Microscopy Fluorescence Quantum dot mental disorders Amyloid aggregation Quantum Dots Biophysics alpha-Synuclein Humans Fluorescent Dyes HeLa Cells |
| Zdroj: | Journal of the American Chemical Society. 131(23) |
| ISSN: | 1520-5126 |
| Popis: | Quantum dots multifunctionalized with the amyloid protein alpha-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only approximately 100 nanoparticles. The combination with a tetracysteine-tagged form of alpha-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells. |
| Databáze: | OpenAIRE |
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