Expression of env gene of bovine leukemia virus in rodent cells
| Autor: | V. Zajac, K. Slavikova, O. Babusikova |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.drug_class
viruses Blotting Western Restriction Mapping Fluorescent Antibody Technique Gene Expression Hamster Enzyme-Linked Immunosorbent Assay Transfection Monoclonal antibody Genes env Chinese hamster Epitope Virus Cell Line Cricetulus Cricetinae Virology Leukemia Virus Bovine medicine Animals Lung Expression vector biology Bovine leukemia virus Gene Products env virus diseases General Medicine biology.organism_classification Molecular biology Blotting Southern DNA Viral |
| Zdroj: | Archives of Virology. 135:201-207 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/bf01309779 |
| Popis: | The BamHI-BamHI fragment of the env gene of bovine leukemia virus (BLV) cloned in pMMEx expression vector was transfected into Chinese hamster cells. Monoclonal antibodies (MAbs) directed against both conformational and sequential epitopes of gp51 of BLV recognized viral polypeptides expressed in hamster cells in Western blotting and enzyme-linked immunosorbent assay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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