Assessment of the mechanics of a tissue-engineered rat trachea in an image-processing environment
| Autor: | Fabio Gava Aoki, Paulo Francisco Guerreiro Cardoso, Thaiane Cristine Evaristo, Henrique Takachi Moriya, Thiago Henrique da Silva, Rogério Pazetti, Marcelo Henrique Valenga, Elenice Deffune, Paulo Manuel Pêgo-Fernandes |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Rat Trachea medicine.medical_treatment Image processing Respiratory physiology Rats Sprague-Dawley Reference Values Medical Illustration Image Processing Computer-Assisted Pressure medicine Animals Rats Wistar Tromethamine Instrumentation Saline lcsh:R5-920 Ventilators Mechanical Decellularization Tissue Engineering business.industry Reproducibility of Results General Medicine Surgery Trachea Transplantation Glucose Respiratory Mechanics Methacholine lcsh:Medicine (General) Airway business Rapid Communication Biomedical engineering medicine.drug |
| Zdroj: | Clinics, Vol 69, Iss 7, Pp 500-503 (2014) Clinics; v. 69 n. 7 (2014); 500-503 Clinics; Vol. 69 Núm. 7 (2014); 500-503 Clinics; Vol. 69 No. 7 (2014); 500-503 Clinics Universidade de São Paulo (USP) instacron:USP SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1807-5932 1980-5322 |
| DOI: | 10.6061/clinics/2014(07)11 |
| Popis: | Made available in DSpace on 2014-09-30T18:18:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-07-01Bitstream added on 2014-10-01T14:03:18Z : No. of bitstreams: 1 S1807-59322014000700500.pdf: 399794 bytes, checksum: d6f90151bfc0b0500b0dfff0ef4f5ccd (MD5) OBJECTIVES: Despite the recent success regarding the transplantation of tissue-engineered airways, the mechanical properties of these grafts are not well understood. Mechanical assessment of a tissue-engineered airway graft before implantation may be used in the future as a predictor of function. The aim of this preliminary work was to develop a noninvasive image-processing environment for the assessment of airway mechanics.METHOD: Decellularized, recellularized and normal tracheas (groups DECEL, RECEL, and CONTROL, respectively) immersed in Krebs-Henseleit solution were ventilated by a small-animal ventilator connected to a Fleisch pneumotachograph and two pressure transducers (differential and gauge). A camera connected to a stereomicroscope captured images of the pulsation of the trachea before instillation of saline solution and after instillation of Krebs-Henseleit solution, followed by instillation with Krebs-Henseleit with methacholine 0.1 M (protocols A, K and KMCh, respectively). The data were post-processed with computer software and statistical comparisons between groups and protocols were performed.RESULTS: There were statistically significant variations in the image measurements of the medial region of the trachea between the groups (two-way analysis of variance [ANOVA], p |
| Databáze: | OpenAIRE |
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