Supramolecular Au(I)-Cu(I) Complexes as New Luminescent Labels for Covalent Bioconjugation
| Autor: | Sergey P. Tunik, Elena V. Grachova, Pavel Yu. Serdobintsev, Alexei S. Melnikov, E. G. Vlakh, Tatiana B. Tennikova, Andrei A. Belyaev, Dmitrii V. Krupenya, Ekaterina Sinitsyna, Vladislav V. Gurzhiy |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Magnetic Resonance Spectroscopy Biomedical Engineering Supramolecular chemistry Pharmaceutical Science Quantum yield Bioengineering 010402 general chemistry Crystallography X-Ray 01 natural sciences Antibodies Coordination Complexes Isothiocyanates medicine Animals Humans Serum Albumin Pharmacology Bioconjugation Luminescent Agents 010405 organic chemistry Chemistry Organic Chemistry Nuclear magnetic resonance spectroscopy Human serum albumin Combinatorial chemistry 0104 chemical sciences Covalent bond Gold Rabbits Trypsin Inhibitor Kunitz Soybean Phosphorescence Luminescence Copper Biotechnology medicine.drug |
| Zdroj: | Bioconjugate chemistry. 27(1) |
| ISSN: | 1520-4812 |
| Popis: | Two new supramolecular organometallic complexes, namely, [Au6Cu2(C2C6H4CHO)6(PPh2C6H4PPh2)3](PF6)2 and [Au6Cu2(C2C6H4NCS)6(PPh2C6H4PPh2)3](PF6)2, with highly reactive aldehyde and isothiocyanate groups have been synthesized and characterized using X-ray crystallography, ESI mass spectrometry, and NMR spectroscopy. The compounds obtained demonstrated bright emission in solution with the excited-state lifetime in microsecond domain both under single- and two-photon excitation. The luminescent complexes were found to be suitable for bioconjugation in aqueous media. In particular, they are able to form the covalent conjugates with proteins of different molecular size (soybean trypsin inhibitor, human serum albumin, rabbit anti-HSA antibodies). The conjugates demonstrated a high level of the phosphorescent emission from the covalently bound label, excellent solubility, and high stability in physiological media. The highest quantum yield, storage stability, and luminance were detected for bioconjugates formed by covalent attachment of the aldehyde-bearing supramolecular Au(I)-Cu(I) complex. The measured biological activity of one of the labeled model proteins clearly showed that introduced label did not prevent the biorecognition and specific protein-protein complex formation that was extremely important for the application of the conjugates in biomolecular detection and imaging. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|