Hsp27 Consolidates Intracellular Redox Homeostasis by Upholding Glutathione in Its Reduced Form and by Decreasing Iron Intracellular Levels
| Autor: | Sophie Virot, Carole Kretz-Remy, André-Patrick Arrigo, Chantal Diaz-Latoud, Sylvain Chaufour, Wance Firdaus |
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| Rok vydání: | 2005 |
| Předmět: |
Physiology
Iron Clinical Biochemistry Glutathione reductase Down-Regulation Dehydrogenase medicine.disease_cause Biochemistry Mice chemistry.chemical_compound Hsp27 Downregulation and upregulation medicine Animals Homeostasis Molecular Biology Heat-Shock Proteins General Environmental Science chemistry.chemical_classification biology General Medicine Cell Biology Glutathione Cell biology Oxidative Stress Enzyme chemistry biology.protein General Earth and Planetary Sciences Hydroxyl radical Oxidation-Reduction Oxidative stress Intracellular |
| Zdroj: | Antioxidants & Redox Signaling. 7:414-422 |
| ISSN: | 1557-7716 1523-0864 |
| Popis: | Small stress proteins [small heat shock proteins (sHsps)] are molecular chaperones that modulate the ability of cells to respond to oxidative stress. The current knowledge concerning the protective mechanism generated by the expression of mammalian heat shock protein-27 (Hsp27) that allows cells to increase their resistance to oxidative stress is presented. We describe the effects mediated by Hsp27 expression toward crucial enzymes such as glucose-6-phosphate dehydrogenase and glutathione reductase that uphold glutathione in its reduced form. New data are presented showing that the expression of sHsps correlates with a drastic decrease in the intracellular level of iron, a catalyzer of hydroxyl radical (OH( . )) generation. A decreased ability of sHsps expressing cells to concentrate iron will therefore end up in a decreased level of oxidized proteins. In addition, we propose a role of Hsp27 in the presentation of oxidized proteins to the proteasome degradation machinery. We also present an analysis of several Hsp27 mutants that suggests that the C-terminal part of this stress protein is essential for its protective activity against oxidative stress. |
| Databáze: | OpenAIRE |
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