Germline genetic patterns underlying familial rheumatoid arthritis, systemic lupus erythematosus and primary Sjögren’s syndrome highlight T cell-initiated autoimmunity
| Autor: | Jing Wang, Songxia Zhou, Jianqun Lin, Marco Matucci-Cerinic, Sini Gao, Chengpeng Zhang, Jingyao Chen, Yukai Wang, Qisheng Lin, Guohong Zhang, Ruiqin Mai, Shaoqi Chen, Daniel E. Furst, Xuezhen Xie |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine T-Lymphocytes T cell Immunology Autoimmunity medicine.disease_cause PTPRC General Biochemistry Genetics and Molecular Biology Arthritis Rheumatoid 03 medical and health sciences 0302 clinical medicine Germline mutation Rheumatology Genetic predisposition Humans Lupus Erythematosus Systemic Immunology and Allergy Medicine Genetic Predisposition to Disease Gene Germ-Line Mutation 030203 arthritis & rheumatology biology business.industry T-cell receptor Family aggregation Sjogren's Syndrome 030104 developmental biology medicine.anatomical_structure biology.protein Female business |
| Zdroj: | Annals of the Rheumatic Diseases. 79:268-275 |
| ISSN: | 1468-2060 0003-4967 |
| Popis: | ObjectivesFamilial aggregation of primary Sjögren’s syndrome (pSS), systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and co-aggregation of these autoimmune diseases (ADs) (also called familial autoimmunity) is well recognised. However, the genetic predisposition variants that explain this clustering remains poorly defined.MethodsWe used whole-exome sequencing on 31 families (9 pSS, 11 SLE, 6 RA and 5 mixed autoimmunity), followed by heterozygous filtering and cosegregation analysis of a family-focused approach to document rare variants predicted to be pathogenic byin silicoanalysis. Potential importance in immune-related processes, gene ontology, pathway enrichment and overlap analyses were performed to prioritise gene sets.ResultsA range from 1 to 50 rare possible pathogenic variants, including 39 variants in immune-related genes across SLE, RA and pSS families, were identified. Among this gene set, regulation of T cell activation (p=4.06×10−7) and T cell receptor (TCR) signalling pathway (p=1.73×10−6) were particularly concentrated, includingPTPRC(CD45),LCK,LAT–SLP76complex genes (THEMIS,LAT,ITK,TEC,TESPA1,PLCL1),DGKD,PRKD1,PAK2andNFAT5, shared across 14 SLE, RA and pSS families. TCR-interactive genesP2RX7,LAG3,PTPN3andLAX1were also detected. Overlap analysis demonstrated that the antiviral immunity geneDUS2variant cosegregated with SLE, RA and pSS phenotypes in an extended family, that variants in the TCR-pathway genesCD45,LCKandPRKD1occurred independently in three mixed autoimmunity families, and that variants inCD36andVWA8occurred in both RA-pSS and SLE-pSS families.ConclusionsOur preliminary results define common genetic characteristics linked to familial pSS, SLE and RA and highlight rare genetic variations in TCR signalling pathway genes which might provide innovative molecular targets for therapeutic interventions for those three ADs. |
| Databáze: | OpenAIRE |
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