Effects of monoclonal antibodies against lymphocyte surface antigens on interleukin 2 excretion by Epstein-Barr virus-specific human T-cell hybridomas
| Autor: | Dennis A. Carson, Mary A. Valentine, Ellen Lakow, John H. Vaughan, Constantine D. Tsoukas |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Interleukin 2
Herpesvirus 4 Human medicine.drug_class Lymphocyte T cell T-Lymphocytes Immunology medicine.disease_cause Monoclonal antibody Antigen hemic and lymphatic diseases medicine Humans Hybridomas biology Lymphoblast Antibodies Monoclonal hemic and immune systems Epstein–Barr virus Virology Molecular biology medicine.anatomical_structure Phenotype Antigens Surface biology.protein Interleukin-2 Antibody medicine.drug |
| Zdroj: | Cellular immunology. 85(1) |
| ISSN: | 0008-8749 |
| Popis: | Monoclonal antibodies were used as probes to study the role of cell surface antigens in the response of Epstein-Barr virus (EBV)-specific human T-T hybridomas to autologous EBV-infected B lymphoblasts. Somatic cell hybrids were generated by fusing EBV-primed peripheral blood T lymphocytes with a mutant clone of the JM human T-lymphoblastoid-cell line. When exposed to autologous EBV-infected B lymphoblasts, the resulting hybrid clones released Interleukin 2 into the culture medium. Incubation of the EBV-infected B cells with two monoclonal antibodies against human Ia-like molecules blocked their ability to trigger the hybridomas. Under the same conditions, monoclonal antibodies against β 2 -microglobulin, and a 45,000 MW surface antigen common to EBV-infected B lymphoblasts, did not alter the capacity of the B cells to stimulate the hybridomas. None of four monoclonal antibodies against surface antigens on the T-cell hybridomas impaired their responsiveness to EBV-infected B lymphoblasts. These results suggest the possibility that naturally occurring or exogenously administered antibodies against Ia molecules might interfere with T-cell regulation of EBV-induced B-cell activation. |
| Databáze: | OpenAIRE |
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