| Autor: |
Moon-Hyeong Seo, Yun Lim, Ji Yul Kim, Youn Hee Jung, Jae Hoon Lee, Min Seok Baek, Je Hyeong Jung, Ho-Youn Kim, Wookbin Lee, Keunwan Park |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Sensors and Actuators B: Chemical. 390:133951 |
| ISSN: |
0925-4005 |
| DOI: |
10.1016/j.snb.2023.133951 |
| Popis: |
Studies on glutamine (Gln) metabolism have illuminated the vital role of Gln in cellular functions and its potential as a biomarker for disease detection. Despite the increasing interest in Gln metabolism, in-depth evaluations are challenging owing to limitations of conventional Gln-measuring methods. Thus, we developed a ligand-induced dimerization-based sensor for Gln, termed Q-SHINE, by splitting a glutamine binding protein into two separate domains. Q-SHINE enables highly accurate and convenient measurement of Gln concentration in bio-fluid samples, and the detection range is optimal for physiological Gln levels. Genetically encoded Q-SHINE sensors could also visualize intracellular Gln levels and quantify cytoplasmic and mitochondrial Gln change in living cells, which enabled detection of various cell responses to extracellular Gln supplement. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect