Near-infrared ratiometric probe with a self-immolative spacer for rapid and sensitive detection of alkaline phosphatase activity and imaging in vivo
| Autor: | Xiangzhu Chen, Kaizheng Liu, Xueyan Zhang, Gui Gao, Shicong Hou, Yuanyuan Zhang, Xiaoqian Huang |
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| Rok vydání: | 2020 |
| Předmět: |
Endogeny
02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Limit of Detection In vivo Animals Humans Environmental Chemistry Carbon Tetrachloride Density Functional Theory Zebrafish Spectroscopy Acetaminophen Fluorescent Dyes Detection limit chemistry.chemical_classification Microscopy Confocal 010401 analytical chemistry Near-infrared spectroscopy Alkaline Phosphatase 021001 nanoscience & nanotechnology Fluorescence Organophosphates 0104 chemical sciences Enzyme Microscopy Fluorescence Models Chemical Linear range chemistry Biophysics Alkaline phosphatase Cattle Carbamates 0210 nano-technology HeLa Cells |
| Zdroj: | Analytica Chimica Acta. 1094:113-121 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2019.10.001 |
| Popis: | Alkaline phosphatase (ALP), an enzyme that catalyzes the hydrolysis of phosphate groups, is closely associated with many diseases, including bone disease, prostate cancer, and diabetes. Thus, new assays for ALP detection in live cells are needed to better understand its role in related biological processes. In this study, we constructed a novel near-infrared ratiometric fluorescent probe for detecting ALP activity with high sensitivity. The probe uses a new self-immolative mechanism that can achieve a rapid response (within 10 min) to ALP, detected as a spectral shift (from 580 to 650 nm). This method effectively avoids issues related to instrument variability, and the near-infrared fluorescence emission (650 nm) makes it more suitable for biological detection. Moreover, the high sensitivity (14-fold enhancement of the fluorescence ratio F650/F580) and low detection limit (0.89 U L−1) for ALP allows the probe to be adapted to complex biological environments. The assay was successfully performed using serum samples with a linear range of ALP of up to 150 U L−1. We used the developed probe to detect and image endogenous ALP in cells with satisfactory results, and we successfully used the probes to detect changes in endogenous ALP levels in zebrafish caused by drug-induced organ damage. |
| Databáze: | OpenAIRE |
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