SCOTfluors: Small, Conjugatable, Orthogonal, and Tunable Fluorophores for In Vivo Imaging of Cell Metabolism

Autor:	Sam Benson, Antonio Fernandez, Nicole D. Barth, Fabio de Moliner, Mathew H. Horrocks, C. Simon Herrington, Jose Luis Abad, Antonio Delgado, Lisa Kelly, Ziyuan Chang, Yi Feng, Miyako Nishiura, Yuichiro Hori, Kazuya Kikuchi, Marc Vendrell
Přispěvatelé:	European Research Council, Abad, José Luís, Abad, José Luís [0000-0002-8343-9611]
Rok vydání:	2019
Předmět:	Motilitat cel·lular Cancer cells Green Fluorescent Proteins Imaging agents Cell motility 010402 general chemistry 01 natural sciences Fluorescent Probes | Hot Paper Fluorescence Super-resolution imaging Neoplasms Metabolites Humans Fluorescent Dyes Cancer Ionophores 010405 organic chemistry Communication General Medicine Metabòlits Communications 0104 chemical sciences 3. Good health Molecular Imaging Microscopy Fluorescence A549 Cells Metabolome Cèl·lules canceroses HeLa Cells
Zdroj:	Dipòsit Digital de la UB Universidad de Barcelona Angewandte Chemie (International Ed. in English) Benson, S, Fernandez, A, Barth, N D, De Moliner, F, Horrocks, M H, Herrington, C S, Abad, J L, Delgado, A, Kelly, L, Chang, Z, Feng, Y, Nishiura, M, Hori, Y, Kikuchi, K & Vendrell, M 2019, ' SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism ', Angewandte Chemie, vol. 131, no. 21, pp. 6985-6989 . https://doi.org/10.1002/ange.201900465 Digital.CSIC. Repositorio Institucional del CSIC instname
DOI:	10.1002/ange.201900465
Popis:	The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metabolites under physiological conditions with high spatial and temporal resolution. Herein, we describe SCOTfluors as novel small-sized multi-colored fluorophores for real-time tracking of essential metabolites in live cells and in vivo and for the acquisition of metabolic profiles from human cancer cells of variable origin. © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. The authors acknowledge funding from Medical Research Scotland (S.B.: 879-2015), MSCA Individual Fellowship (A.F.: 704912), OPTIMA (N.D.B.: EP/L016559/1), Wellcome Trust Sir Henry Dale Fellowship (Y.F.: 100104/Z/12/Z) and the Spanish Ministry of Science, Innovation and Universities (J.L.A, A.D.: CTQ2017-85378-R). M.V. acknowledges funds from ERC Consolidator Grant (771443), Biotechnology and Biological Sciences Research Council (BB/M025160/1) and the Royal Society (IEC\R3\170132). The authors thank the technical support from the Flow Cytometry and the Confocal Advanced Light Microscopy units at the University of Edinburgh.
Databáze:	OpenAIRE
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