Diagnosis of Meningococcal Infection Using Internally Controlled Multiplex Real-Time PCR
| Autor: | Eva Hong, Muhamed-Kheir Taha, Ala-Eddine Deghmane |
|---|---|
| Přispěvatelé: | Infections Bactériennes Invasives, Institut Pasteur [Paris], This work was supported by the Institut Pasteur, Paris., Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Multiplex real-time PCR medicine.drug_class 030106 microbiology Antibiotics Neisseria meningitidis Clinical specimens medicine.disease_cause MESH: Meningococcal Infections MESH: Neisseria meningitidis 03 medical and health sciences 0302 clinical medicine Phocine herpes virus medicine Multiplex 030212 general & internal medicine Gene MESH: Bacterial Proteins MESH: Humans MESH: Molecular Typing MESH: Multiplex Polymerase Chain Reaction business.industry MESH: Real-Time Polymerase Chain Reaction medicine.disease Virology DNA extraction DNA isolation MESH: DNA Bacterial [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Serogroups 3. Good health Internal quality Real-time polymerase chain reaction crgA business Meningitis |
| Zdroj: | Neisseria meningitidis Neisseria meningitidis, 1969, Humana Press, New York, NY, pp.17-31, 2019, Methods in Molecular Biology, 978-1-4939-9201-0. ⟨10.1007/978-1-4939-9202-7_2⟩ Methods in Molecular Biology ISBN: 9781493992010 |
| DOI: | 10.1007/978-1-4939-9202-7_2⟩ |
| Popis: | International audience; Neisseria meningitidis (Nm) is a leading cause of invasive infections associated with high mortality and morbidity, notably meningitis and septicemia. Etiological rapid diagnosis is key for the preventive management of invasive meningococcal disease (IMD). However, conventional methods for diagnosis are timeconsuming and could be hampered by the difficulties in culturing the isolates from clinical specimens especially due to early antibiotic treatment. Therefore, sensitive, specific and rapid non-culture-based methods are valuable for early diagnosis, effective therapy, and prevention. Here we describe a real-time PCR multiplex assays for the detection of Nm targeting the meningococcal-specific gene crgA, coding for a LysR-like transcriptional regulator, and six serogroup-specific (A, B, C, W, X, Y) Nm capsular genes, using a Qiagen column-based method for the optimum isolation of DNA from clinical specimens. Internal quality controls were included to monitor extraction of DNA, inhibition and the technical validation of the PCR as well. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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