Assistance of human serum albumin to photo-sensitized inactivation of Saccharomyces cerevisiae with axially pyridinio-bonded P-porphyrins
| Autor: | Shigetoshi Okazaki, Haruhiko Yokoi, Masahide Yasuda, Yuya Kai, Jin Matsumoto |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Porphyrins
Light Stereochemistry Saccharomyces cerevisiae Biophysics Serum albumin 02 engineering and technology 010402 general chemistry 01 natural sciences Medicinal chemistry medicine Humans Radiology Nuclear Medicine and imaging Photosensitizer Solubility Serum Albumin Alkyl chemistry.chemical_classification Photosensitizing Agents Radiation Singlet Oxygen Radiological and Ultrasound Technology biology Chemistry Water Half-life 021001 nanoscience & nanotechnology Human serum albumin biology.organism_classification Yeast 0104 chemical sciences body regions Microscopy Fluorescence biology.protein 0210 nano-technology Half-Life Protein Binding medicine.drug |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 161:279-283 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/j.jphotobiol.2016.05.024 |
| Popis: | As singlet-oxygen ((1)O2) sensitizer, water-soluble P-porphyrins (1) were prepared by the modification of axial ligands of tetraphenylporphyrinatophosphorus by N-alkyl-pyridinium group to give bis[3-(1-alkyl-4-pyridinio)propoxo]tetraphenylporphyrinatophosphorus(V) (alkyl=hexyl (1a) and butyl (1b)) and bis[5-(3-alkyl-1-pyridinio)-3-oxapentyloxo] tetraphenylporphyrinatophosphorus(V) (alkyl=hexyl (1c), butyl (1d), and ethyl (1e)). The quantum yields (ΦΔ) for the formation of (1)O2 were extremely high (e.g. ΦΔ=0.88 (1a) and 0.87 (1c)). The 1 could bind human serum albumin (HSA) with high binding constants to produce a complex of HSA with 1 (1/HSA). Here, inactivation of Saccharomyces cerevisiae (yeast) was examined using the complex of HSA (400nM) with 1 (20-50nM) under visible-light irradiation. The bactericidal activity of 1 was evaluated by half-life (T1/2 in min) which was time required to be reduced to one-half initial concentration of yeasts. Under irradiation in the presence of HSA, minimum concentrations ([P]) of 1 were adjusted as T1/2 became the values among 0-120min. The [P] values were determined to be 20nM for 1a, 30nM for 1b, and 50nM for 1c-1e. The 1/HSA sterilized yeast with 12-36min of T1/2 when the concentration of 1 was set to [P]. In the photo-activation in the absence of HSA at the concentration of [P] of 1, however, the T1/2 values of 1a, 1b, 1d, and 1e were >120min and T1/2 of 1c was 44min. Thus, the complexation of 1 with HSA apparently enhanced the bactericidal activity of 1. This is the first finding on the assistance of HSA to the photo-inactivation of yeast cell by porphyrins. |
| Databáze: | OpenAIRE |
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