Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1
| Autor: | Joanna Kaminska, Teresa Żołądek, Magdalena Sędek, Monika Wysocka-Kapcinska |
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| Rok vydání: | 2007 |
| Předmět: |
Saccharomyces cerevisiae Proteins
viruses Molecular Sequence Data Biophysics Active Transport Cell Nucleus Importin Saccharomyces cerevisiae Actin-binding protein environment and public health Biochemistry Fungal Proteins Nuclear localization signal Structural Biology Genes Reporter Genetics medicine Nuclear protein Nuclear export signal Molecular Biology Actin Cell Nucleus Nuclear Export Signals biology Microfilament Proteins Pan1 Cell Biology Yeast Cell biology medicine.anatomical_structure Cytoplasm biology.protein lipids (amino acids peptides and proteins) Nucleus Nuclear localization sequence |
| Zdroj: | FEBS letters. 581(28) |
| ISSN: | 0014-5793 |
| Popis: | Pan1 is an actin patch-associated protein involved in endocytosis. Our studies revealed that in oleate-grown cells Pan1 is located in the nucleus as well as in patches. One of three putative nuclear localization signals (NLS) of Pan1, NLS2, directed beta-galactosidase (beta-gal) to the nucleus. However, GFP-Pan1(886-1219), containing NLS2, was found in the cytoplasm indicating that it may contain a nuclear export signal (NES). A putative Pan1 NES, overlapping with NLS3, re-addressed NLS(H2B)-NES/NLS3-beta-gal from the nucleus to the cytoplasm. Inactivation of the NES allowed NLS3 to be effective. Thus, Pan1 contains functional NLSs and a NES and appears to shuttle in certain circumstances. |
| Databáze: | OpenAIRE |
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