Evaluation of API Coryne in comparison with conventional methods for identifying coryneform bacteria

Autor:	Jean Fleurette, F Allard, Daniel Monget, J M Boeufgras, C Courtier, Willy Hansen, Jean Freney, M T Duperron
Rok vydání:	1991
Předmět:	Microbiology (medical) Identification methods Bacteriological Techniques Evaluation Studies as Topic Corynebacterium Humans Code book Biology biology.organism_classification Bacteria Research Article Microbiology
Zdroj:	Journal of Clinical Microbiology. 29:38-41
ISSN:	1098-660X 0095-1137
DOI:	10.1128/jcm.29.1.38-41.1991
Popis:	A study was performed to evaluate a new manual miniaturized system, API Coryne (API-bioMérieux, Inc., La Balme les Grottes, France), in which conventional biochemical methods were used to identify 240 isolates of coryneform and related bacteria. A total of 40% of the isolates were excluded from the study because they could not be identified by conventional methods. Identifications of the 240 isolates obtained with API Coryne showed a 97.6% concordance with conventional methods (79% after 24 h of incubation, 21% after 48 h of incubation): 158 (65.8%) isolates were identified with no further testing, and extra testing was required for 76 (31.8%) isolates. In three (1.2%) cases, the organisms did not correspond to any key in the code book and could not be identified by the computer service of the manufacturer. Only three (1.2%) strains were misidentified. The system was shown to be reliable and rapid when compared with standard identification methods.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3417e7ac4b608540e6fc5b259a7e664 https://doi.org/10.1128/jcm.29.1.38-41.1991 Zobrazit plný text záznamu