Design of an optimal promoter involved in the heat-induced transcriptional pathway in Arabidopsis, soybean, rice, and maize
| Autor: | Kaoru Urano, Kyouko Yoshiwara, Norihito Kanamori, Hitoshi Sakakibara, Chihiro Noda, Yoshiharu Y. Yamamoto, Kazuo Shinozaki, Shingo Goto, Kazuko Yamaguchi-Shinozaki, Mikiko Kojima, Takuhiro Yoshida, Yuko Tokoro, Hiroko Urawa, Takuya Ogata, Kyonoshin Maruyama, Satoshi Iuchi, Tetsuya Sakurai, Yuta Takaki |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hot Temperature Transcription Genetic Cellular differentiation Arabidopsis Plant Science Biology 01 natural sciences Zea mays 03 medical and health sciences Gene Expression Regulation Plant Gene expression Genetics Heat shock Promoter Regions Genetic Transcription factor Gene Plant Proteins Molecular breeding Promoter Oryza Cell Biology biology.organism_classification 030104 developmental biology Soybeans Heat-Shock Response 010606 plant biology & botany Transcription Factors |
| Zdroj: | SC30201707060019 Free Access Article C30201611220002_284.pdf有 |
| ISSN: | 0960-7412 |
| DOI: | 10.1111/tpj.13420 |
| Popis: | Interactions between heat shock (HS) factors (HSFs) and heat shock response elements (HSEs) are important during the heat shock response (HSR) of flora and fauna. Moreover, plant HSFs that are involved in heat stress are also involved in abiotic stresses such as dehydration and cold as well as development, cell differentiation and proliferation. Because the specific combination of HSFs and HSEs involved in plants under heat stress remains unclear, the mechanism of their interaction has not yet been utilized in molecular breeding of plants for climate change. For the study reported herein, we compared the sequences of HS-inducible genes and their promoters in Arabidopsis, soybean, rice and maize and then designed an optimal HS-inducible promoter. Our analyses suggest that, for the four species, the abscisic acid-independent, HSE/HSF-dependent transcriptional pathway plays a major role in HS-inducible gene expression. We found that an 18-bp sequence that includes the HSE has an important role in the HSR, and that those sequences could be classified as representative of monocotyledons or dicotyledons. With the HS-inducible promoter designed based on our bioinformatic predictions, we were able to develop an optimal HS-specific inducible promoter for seedlings or single cells in roots. These findings demonstrate the utility of our HS-specific inducible promoter, which we expect will contribute to molecular breeding efforts and cell-targeted gene expression in specific plant tissues. |
| Databáze: | OpenAIRE |
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