Stem Cell Derived Retinal Pigment Epithelium
| Autor: | Arthur A.B. Bergen, L. A. E. Catsburg, Vivi M. Heine, Reinier O. Schlingemann, J. van Meurs, Perry D. Moerland, Céline Koster, J.B. ten Brink, Theo G. M. F. Gorgels, Anna Bennis, J. G. Jacobs |
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| Přispěvatelé: | ANS - Complex Trait Genetics, AR&D - Amsterdam Reproduction & Development, Graduate School, ACS - Amsterdam Cardiovascular Sciences, ANS - Amsterdam Neuroscience, Ophthalmology, Other departments, APH - Personalized Medicine, APH - Methodology, Epidemiology and Data Science, Human Genetics, ACS - Atherosclerosis & ischemic syndromes, Complex Trait Genetics, Amsterdam Neuroscience - Complex Trait Genetics, Netherlands Institute for Neuroscience (NIN), Amsterdam Neuroscience - Cellular & Molecular Mechanisms, Pediatric surgery, Human genetics, Amsterdam Reproduction & Development (AR&D), MUMC+: *AB Onderzoekers (9), Oogheelkunde, RS: FHML non-thematic output |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Cellular differentiation Gene Expression 0302 clinical medicine OXIDATIVE STRESS Induced pluripotent stem cell Age related macular degeneration Genetics Pigmentation Cell Polarity Cell Differentiation Cell biology medicine.anatomical_structure VISUAL FUNCTION DIFFERENTIATION Human embryonic stem cells Stem cell Visual phototransduction Signal Transduction Pluripotent Stem Cells MELATONIN Biology Article Cell Line PHAGOCYTOSIS 03 medical and health sciences AGE medicine Cell Adhesion Journal Article Humans RPE CELLS Transcriptomics Retinal pigment epithelium TRANSPLANTATION Gene Expression Profiling Cell Biology MACULAR DEGENERATION Embryonic stem cell eye diseases Gene expression profiling Transplantation 030104 developmental biology Tissue Array Analysis 030221 ophthalmology & optometry Cell replacement therapy sense organs INTEGRIN-LINKED KINASE Biomarkers Transcription Factors |
| Zdroj: | STEM CELL REVIEWS AND REPORTS, 13(5), 659-669. Humana Press Bennis, A, Jacobs, J G, Catsburg, L A E, ten Brink, J B, Koster, C, Schlingemann, R O, van Meurs, J, Gorgels, T G M F, Moerland, P D, Heine, V M & Bergen, A A 2017, ' Stem Cell Derived Retinal Pigment Epithelium : The Role of Pigmentation as Maturation Marker and Gene Expression Profile Comparison with Human Endogenous Retinal Pigment Epithelium ', Stem Cell Reviews and Reports, vol. 13, no. 5, pp. 659-669 . https://doi.org/10.1007/s12015-017-9754-0 Stem cell reviews and reports, 13(5), 659-669. Humana Press Stem Cell Reviews and Reports, 13, 659-669. Humana Press Stem Cell Reviews Bennis, A, Jacobs, J G, Catsburg, L A E, Ten Brink, J B, Koster, C, Schlingemann, R O, van Meurs, J, Gorgels, T G M F, Moerland, P D, Heine, V M & Bergen, A A 2017, ' Stem Cell Derived Retinal Pigment Epithelium : The Role of Pigmentation as Maturation Marker and Gene Expression Profile Comparison with Human Endogenous Retinal Pigment Epithelium ', Stem cell reviews and reports, vol. 13, no. 5, pp. 659-669 . https://doi.org/10.1007/s12015-017-9754-0 Stem Cell Reviews and Reports, 13(5), 659-669. Humana Press Stem Cell Reviews and Reports, 13(5), 659-669. Springer |
| ISSN: | 1558-6804 2629-3269 2629-3277 1550-8943 |
| DOI: | 10.1007/s12015-017-9754-0 |
| Popis: | In age-related macular degeneration (AMD) the retinal pigment epithelium (RPE) deteriorates, leading to photoreceptor decay and severe vision loss. New therapeutic strategies aim at RPE replacement by transplantation of pluripotent stem cell (PSC)-derived RPE. Several protocols to generate RPE have been developed where appearance of pigmentation is commonly used as indicator of RPE differentiation and maturation. It is, however, unclear how different pigmentation stages reflect developmental stages and functionality of PSC-derived RPE cells. We generated human embryonic stem cell-derived RPE (hESC-RPE) cells and investigated their gene expression profiles at early pigmentation (EP) and late pigmentation (LP) stages. In addition, we compared the hESC-RPE samples with human endogenous RPE. We used a common reference design microarray (44 K). Our analysis showed that maturing hESC-RPE, upon acquiring pigmentation, expresses markers specific for human RPE. Interestingly, our analysis revealed that EP and LP hESC-RPE do not differ much in gene expression. Our data further showed that pigmented hESC-RPE has a significant lower expression than human endogenous RPE in the visual cycle and oxidative stress pathways. In contrast, we observed a significantly higher expression of pathways related to the process adhesion-to-polarity model that is typical of developing epithelial cells. We conclude that, in vitro, the first appearance of pigmentation hallmarks differentiated RPE. However, further increase in pigmentation does not result in much significant gene expression changes and does not add important RPE functionalities. Consequently, our results suggest that the time span for obtaining differentiated hESC-RPE cells, that are suitable for transplantation, may be greatly reduced. Electronic supplementary material The online version of this article (doi:10.1007/s12015-017-9754-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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