Identification and characterization of sex-linked proteins of Schistosoma mansoni
| Autor: | Maldonado Júnior A, de Simone Sg |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Microbiology (medical) lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Octoxynol Blotting Western Detergents lcsh:QR1-502 tegumental proteins amphiphilic proteins lcsh:Microbiology Polyethylene Glycols Silver stain Sex Factors Antigen Animals Polyacrylamide gel electrophoresis Triton X-114 Gel electrophoresis biology Nectomys squamipes Membrane Proteins Schistosoma mansoni biology.organism_classification Molecular biology Staining Blot BH and RJ isolates Antigens Surface sex-linked proteins Electrophoresis Polyacrylamide Gel Female |
| Zdroj: | Scopus-Elsevier Memórias do Instituto Oswaldo Cruz., Vol 86, Iss 1, Pp 31-36 (1991) Memórias do Instituto Oswaldo Cruz, Volume: 86, Issue: 1, Pages: 31-36, Published: MAR 1991 |
| ISSN: | 0074-0276 |
| DOI: | 10.1590/s0074-02761991000100006 |
| Popis: | The proteins of adults worms (male and female) of two isolates (BH and RJ) of Schistosoma mansoni were extracted using Triton X-114 phase separation. The SDS-polyacrilamide gel electrophoresis profiles of the three phases (detergent, aqueous and insoluble proteins) obtained were compared after Coomassie blue and silver staining, surface radioiodination and Western blotting. No major differences were detected between the 2 isolates. Of the 25 or more proteins which partitioned into the detergent phase, only about 8 proteins could be surface radiodinated on live adult worms. A comparison was also made between the profiles of male and females worms, isolated from bisexually infected mice. Two major female-specific and one male-specific band were detected by silver and/or Coomassie staining. The female bands, 32 KDa and 18 KDa, partitioned into the detergent and aqueous phase, respectively. The male-specific band of 42 KDa remained in the insoluble phase. Antigenic differences between male and females proteins were detected by Western blotting using a sera from infected Nectomys squamipes. |
| Databáze: | OpenAIRE |
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